ELISA/assay

Human Bone marrow stromal antigen 2, BST2 ELISA Kit

MBS925956

48-Strip-Wells

510 USD

ELISA Kit

Human Bone marrow stromal antigen 2, BST2 ELISA Kit

bone marrow stromal cell antigen 2

Human Bone marrow stromal antigen 2 (BST2) ELISA kit; CD317; tetherin; bone marrow stromal cell antigen 2

bone marrow stromal antigen 2; Bone marrow stromal antigen 2; bone marrow stromal antigen 2; BST-2; NPC-A-7; HM1.24 antigen; bone marrow stromal cell antigen 2; HM1.24 antigen; Tetherin; CD_antigen: CD317

BST2

BST2; BST2; CD317; TETHERIN; BST-2

BST2_HUMAN

Human

180

This assay has high sensitivity and excellent specificity for detection of Human BST2. No significant cross-reactivity or interference between Human BST2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: 3.9 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Immunity. Protein Biological Process 3: Antiviral defense

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BST2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BST2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BST2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BST2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

4757876

NP_004326.1

NM_004335.3

Q10589

19,769 Da

Bone marrow stromal cells are involved in the growth and development of B-cells. The specific function of the protein encoded by the bone marrow stromal cell antigen 2 is undetermined; however, this protein may play a role in pre-B-cell growth and in rheumatoid arthritis. [provided by RefSeq, Jul 2008]

BST2: IFN-induced antiviral host restriction factor which efficiently blocks the release of diverse mammalian enveloped viruses by directly tethering nascent virions to the membranes of infected cells. Acts as a direct physical tether, holding virions to the cell membrane and linking virions to each other. The tethered virions can be internalized by endocytosis and subsequently degraded or they can remain on the cell surface. In either case, their spread as cell-free virions is restricted. Its target viruses belong to diverse families, including retroviridae: human immunodeficiency virus type 1 (HIV-1), human immunodeficiency virus type 2 (HIV-2), simian immunodeficiency viruses (SIVs), equine infectious anemia virus (EIAV), feline immunodeficiency virus (FIV), prototype foamy virus (PFV), Mason- Pfizer monkey virus (MPMV), human T-cell leukemia virus type 1 (HTLV-1), Rous sarcoma virus (RSV) and murine leukemia virus (MLV), flavivirideae: hepatitis C virus (HCV), filoviridae: ebola virus (EBOV) and marburg virus (MARV), arenaviridae: lassa virus (LASV) and machupo virus (MACV), herpesviridae: kaposis sarcoma- associated herpesvirus (KSHV), rhabdoviridae: vesicular stomatitis virus (VSV), orthomyxoviridae: influenza A virus, and paramyxoviridae: nipah virus. Can inhibit cell surface proteolytic activity of MMP14 causing decreased activation of MMP15 which results in inhibition of cell growth and migration. Can stimulate signaling by LILRA4/ILT7 and consequently provide negative feedback to the production of IFN by plasmacytoid dendritic cells in response to viral infection. Plays a role in the organization of the subapical actin cytoskeleton in polarized epithelial cells. Belongs to the tetherin family. Protein type: Membrane protein, GPI anchor; Membrane protein, integral; Cell development/differentiation. Chromosomal Location of Human Ortholog: 19p13.1. Cellular Component: Golgi apparatus; cell surface; membrane; integral to plasma membrane; late endosome; apical plasma membrane; cytoplasm; plasma membrane; lipid raft. Molecular Function: metalloendopeptidase inhibitor activity; protein binding; signal transducer activity; protein homodimerization activity. Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; B cell activation; response to virus; cytokine and chemokine mediated signaling pathway; multicellular organismal development; humoral immune response; cell proliferation; negative regulation of plasmacytoid dendritic cell cytokine production; cell-cell signaling; negative regulation of viral genome replication; regulation of actin cytoskeleton organization and biogenesis; innate immune response; negative regulation of cell growth; defense response to virus; negative regulation of cell migration

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800