Human Interferon-induced protein with tetratricopeptide repeats 1, IFIT1 ELISA Kit | MyBioSource MBS925861 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Interferon-induced protein with tetratricopeptide repeats 1, IFIT1 ELISA Kit

catalog :

MBS925861

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS925861

products type :

ELISA Kit

products full name :

Human Interferon-induced protein with tetratricopeptide repeats 1, IFIT1 ELISA Kit

products short name :

interferon-induced protein with tetratricopeptide repeats 1

products name syn :

Human Interferon-induced protein with tetratricopeptide repeats 1 (IFIT1) ELISA kit; G10P1; GARG-16; IFI-56; IFI56; IFNAI1; ISG56; RNM561; Interferon; alpha-inducible protein (MW 56kD) ; interferon-induced protein 56; interferon-induced protein with tetratricopeptide repeats 1

other names :

interferon-induced protein with tetratricopeptide repeats 1 isoform 3; Interferon-induced protein with tetratricopeptide repeats 1; interferon-induced protein with tetratricopeptide repeats 1; interferon-inducible mRNA 561; interferon-induced 56 kDa protein; interferon, alpha-inducible protein (MW 56kD); interferon-induced protein with tetratricopeptide repeats 1; Interferon-induced 56 kDa protein; IFI-56K; P56

products gene name :

IFIT1

other gene names :

IFIT1; IFIT1; C56; P56; G10P1; IFI56; ISG56; IFI-56; IFIT-1; IFNAI1; RNM561; IFI-56K; G10P1; IFI56; IFNAI1; ISG56; IFIT-1; IFI-56K; P56

uniprot entry name :

IFIT1_HUMAN

reactivity :

Human

sequence length :

447

specificity :

This assay has high sensitivity and excellent specificity for detection of human IFIT1. No significant cross-reactivity or interference between human IFIT1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of human IFIT1 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IFIT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFIT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IFIT1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFIT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

400286381

ncbi acc num :

NP_001257857.1

ncbi gb acc num :

NM_001270928.1

uniprot acc num :

P09914

ncbi mol weight :

55,360 Da

ncbi pathways :

Antiviral Mechanism By IFN-stimulated Genes Pathway (530760); Cytokine Signaling In Immune System Pathway (366171); Hepatitis C Pathway (173973); Hepatitis C Pathway (173907); Herpes Simplex Infection Pathway (377873); Herpes Simplex Infection Pathway (377865); ISG15 Antiviral Mechanism Pathway (530761); Immune System Pathway (106386); Interferon Signaling Pathway (187103); Interferon Alpha/beta Signaling Pathway (187104)

ncbi summary :

This gene encodes a protein containing tetratricopeptide repeats that was originally identified as induced upon treatment with interferon. The encoded protein may inhibit viral replication and translational initiation. This gene is located in a cluster on chromosome 10 with five other closely related genes. There is a pseudogene for this gene on chromosome 13. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Aug 2012]

uniprot summary :

IFIT1: IFN-induced antiviral protein which inhibits expression of viral messenger RNAs lacking 2 -O-methylation of the 5 cap. The ribose 2 -O-methylation would provide a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Viruses evolved several ways to evade this restriction system such as encoding their own 2 -O-methylase for their mRNAs or by stealing host cap containing the 2 -O- methylation (cap snatching mechanism). Exhibits antiviral activity against several viruses including human papilloma and hepatitis C viruses. Belongs to the IFIT family. Chromosomal Location of Human Ortholog: 10q23.31. Cellular Component: cytoplasm; cytosol. Molecular Function: protein binding; RNA binding. Biological Process: intracellular transport of viral proteins in host cell; positive regulation of viral genome replication; negative regulation of helicase activity; negative regulation of viral genome replication; response to virus; cytokine and chemokine mediated signaling pathway; negative regulation of defense response to virus by host; negative regulation of protein binding; defense response to virus

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage