Human WNT1-inducible-signaling pathway protein 2, WISP2 ELISA Kit | MyBioSource MBS925855 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human WNT1-inducible-signaling pathway protein 2, WISP2 ELISA Kit

catalog :

MBS925855

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS925855

products type :

ELISA Kit

products full name :

Human WNT1-inducible-signaling pathway protein 2, WISP2 ELISA Kit

products short name :

WNT1 inducible signaling pathway protein 2

products name syn :

Human WNT1-inducible-signaling pathway protein 2 (WISP2) ELISA kit; CCN5; CT58; CTGF-L; OTTHUMP00000063227; connective tissue growth factor-like protein; wnt-1 signaling pathway protein 2; WNT1 inducible signaling pathway protein 2

other names :

WNT1-inducible-signaling pathway protein 2; WNT1-inducible-signaling pathway protein 2; WNT1-inducible-signaling pathway protein 2; WISP-2; CCN family member 5; wnt-1 signaling pathway protein 2; connective tissue growth factor-like protein; connective tissue growth factor-related protein 58; WNT1 inducible signaling pathway protein 2; CCN family member 5; Connective tissue growth factor-like protein; CTGF-L; Connective tissue growth factor-related protein 58

products gene name :

WISP2

other gene names :

WISP2; WISP2; CCN5; CT58; CTGF-L; CCN5; CT58; CTGFL; UNQ228/PRO261; WISP-2; CTGF-L

uniprot entry name :

WISP2_HUMAN

reactivity :

Human

sequence length :

250

specificity :

This assay has high sensitivity and excellent specificity for detection of human WISP2. No significant cross-reactivity or interference between human WISP2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Quantitative Sandwich. Sensitivity: Typically less than 0.039 ng/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for WISP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WISP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WISP2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WISP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4507923

ncbi acc num :

NP_003872.1

ncbi gb acc num :

NM_003881.2

uniprot acc num :

O76076

ncbi mol weight :

26,825 Da

ncbi summary :

This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like (CT) domain. The encoded protein lacks the CT domain which is implicated in dimerization and heparin binding. It is 72% identical to the mouse protein at the amino acid level. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Its expression in colon tumors is reduced while the other two WISP members are overexpressed in colon tumors. It is expressed at high levels in bone tissue, and may play an important role in modulating bone turnover. [provided by RefSeq, Jul 2008]

uniprot summary :

WISP2: May play an important role in modulating bone turnover. Promotes the adhesion of osteoblast cells and inhibits the binding of fibrinogen to integrin receptors. In addition, inhibits osteocalcin production. Belongs to the CCN family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 20q13.12. Cellular Component: proteinaceous extracellular matrix; extracellular space; plasma membrane. Molecular Function: integrin binding; heparin binding; insulin-like growth factor binding. Biological Process: cell-cell signaling; regulation of cell growth; signal transduction; cell adhesion

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage