Human V-set and immunoglobulin domain-containing protein 4, VSIG4 ELISA Kit | MyBioSource MBS925833 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human V-set and immunoglobulin domain-containing protein 4, VSIG4 ELISA Kit

catalog :

MBS925833

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS925833

products type :

ELISA Kit

products full name :

Human V-set and immunoglobulin domain-containing protein 4, VSIG4 ELISA Kit

products short name :

V-set and immunoglobulin domain containing 4

products name syn :

Human V-set and immunoglobulin domain-containing protein 4 (VSIG4) ELISA kit; RP6-159A1.1; CRIg; Z39IG; Ig superfamily protein; complement receptor of the immunoglobulin superfamily; V-set and immunoglobulin domain containing 4

other names :

V-set and immunoglobulin domain-containing protein 4 isoform 2; V-set and immunoglobulin domain-containing protein 4; V-set and immunoglobulin domain-containing protein 4; protein Z39Ig; Ig superfamily protein; complement receptor of the immunoglobulin superfamily; V-set and immunoglobulin domain containing 4; Protein Z39Ig

products gene name :

VSIG4

other gene names :

VSIG4; VSIG4; CRIg; Z39IG; CRIg; Z39IG; UNQ317/PRO362

uniprot entry name :

VSIG4_HUMAN

reactivity :

Human

sequence length :

305

specificity :

This assay has high sensitivity and excellent specificity for detection of human VSIG4. No significant cross-reactivity or interference between human VSIG4 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: The minimum detectable dose of human VSIG4 is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VSIG4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VSIG4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VSIG4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VSIG4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

154426280

ncbi acc num :

NP_001093901.1

ncbi gb acc num :

NM_001100431.1

uniprot acc num :

Q9Y279

ncbi mol weight :

43,987 Da

ncbi summary :

This gene encodes a v-set and immunoglobulin-domain containing protein that is structurally related to the B7 family of immune regulatory proteins. The encoded protein may be a negative regulator of T-cell responses. This protein is also a receptor for the complement component 3 fragments C3b and iC3b. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010]

uniprot summary :

VSIG4: Phagocytic receptor, strong negative regulator of T-cell proliferation and IL2 production. Potent inhibitor of the alternative complement pathway convertases. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Receptor, misc.; Membrane protein, integral. Chromosomal Location of Human Ortholog: Xq12-q13.3. Cellular Component: integral to membrane. Molecular Function: protein binding. Biological Process: negative regulation of T cell proliferation; complement activation, alternative pathway; negative regulation of interleukin-2 production

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage