ELISA/assay

Human Fibronectin type III domain-containing protein 5, FNDC5 ELISA Kit

MBS925506

48-Strip-Wells

510 USD

ELISA Kit

Human Fibronectin type III domain-containing protein 5, FNDC5 ELISA Kit

fibronectin type III domain containing 5

Human Fibronectin type III domain-containing protein 5 (FNDC5) ELISA kit; FRCP2; ; fibronectin type III domain containing 5

fibronectin type III domain-containing protein 5 isoform 3 preproprotein; Fibronectin type III domain-containing protein 5; fibronectin type III domain-containing protein 5; fibronectin type III repeat-containing protein 2; fibronectin type III domain containing 5; Fibronectin type III repeat-containing protein 2Cleaved into the following chain:Irisin

FNDC5

FNDC5; FNDC5; FRCP2; irisin; FRCP2

FNDC5_HUMAN

Human

181

This assay has high sensitivity and excellent specificity for detection of human FNDC5. No significant cross-reactivity or interference between human FNDC5 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 7.8 ng/ml -500 ng/ml. Sensitivity: The minimum detectable dose of human FNDC5 is typically less than 1.95 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FNDC5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FNDC5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FNDC5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FNDC5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

511093995

NP_001165411.2

NM_001171940.1

Q8NAU1

23,659 Da

This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

FNDC5: Irisin: mediates beneficial effects of muscular exercise. Induces browning of white adipose tissue by stimulating UCP1 expression, at least in part, via the nuclear receptor PPARA. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 1p35.1. Cellular Component: peroxisomal membrane; endoplasmic reticulum; integral to membrane; extracellular region; plasma membrane. Molecular Function: hormone activity. Biological Process: response to muscle activity

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800