catalog number :
MBS925438
products type :
ELISA Kit
products full name :
Human Leukocyte cell-derived chemotaxin-2, LECT2 ELISA Kit
products short name :
[leukocyte cell-derived chemotaxin 2]
products name syn :
[Human Leukocyte cell-derived chemotaxin-2 (LECT2) ELISA kit; MGC126628; chm-II; chm2; chondromodulin-II; leukocyte cell-derived chemotaxin 2]
other names :
[leukocyte cell-derived chemotaxin-2; Leukocyte cell-derived chemotaxin-2; leukocyte cell-derived chemotaxin-2; chondromodulin-II; leukocyte cell-derived chemotaxin 2]
products gene name :
[LECT2]
other gene names :
[LECT2; LECT2; chm2; chm-II; LECT-2; hLECT2]
uniprot entry name :
LECT2_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human LDHC. No significant cross-reactivity or interference between human LDHC and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Tissue Homogenates, Urine. Assay Type: Quantitative Sandwich. Detection Range: 6.25 mU/ml-400 mU/ml. Sensitivity: 1.56 mU/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LDHC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LDHC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LDHC is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LDHC bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_002293.2
ncbi gb acc num :
NM_002302.2
ncbi mol weight :
16,390 Da
ncbi pathways :
C-MYB Transcription Factor Network Pathway (138073)
ncbi summary :
This gene encodes a secreted, 16 kDa protein that acts as a chemotactic factor to neutrophils and stimulates the growth of chondrocytes and osteoblasts. This protein has high sequence similarity to the chondromodulin repeat regions of the chicken myb-induced myeloid 1 protein. A polymorphism in this gene may be associated with rheumatoid arthritis. [provided by RefSeq, Jul 2008]
uniprot summary :
LECT2: Has a neutrophil chemotactic activity. Also a positive regulator of chondrocyte proliferation. Belongs to the LECT2/MIM-1 family. Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 5q31.1. Cellular Component: extracellular space; cytoplasm. Molecular Function: identical protein binding. Biological Process: negative regulation of Wnt receptor signaling pathway; chemotaxis; skeletal development
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells