ELISA/assay

Rat apolipoprotein B-48, ApoB48 ELISA Kit

MBS925341

48-Strip-Wells

510 USD

ELISA Kit

Rat apolipoprotein B-48, ApoB48 ELISA Kit

apolipoprotein B-48 (ApoB48)

Rat apolipoprotein B-48 (ApoB48) ELISA kit; apolipoprotein B-48 (ApoB48)

apolipoprotein B-100; Apolipoprotein B-100; apolipoprotein B-100; apolipoprotein B PI; apolipoprotein B-48; apolipoprotein B (including Ag(x) antigen); apolipoprotein B

ApoB48

Apob; Apob; Aa1064; Ac1-060; ApoB-48; ApoB-100; Apo B-100; Aa1064; Ac1-060; Apo B-100; Apo B-48

APOB_RAT

Rat

4506

This assay has high sensitivity and excellent specificity for detection of rat ApoB48. No significant cross-reactivity or interference between rat ApoB48 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Competitive. Detection Range: 0.312 ug/ml-20 ug/ml. Sensitivity: 0.078 ug/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with ApoB48. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for ApoB48. The competitive inhibition reaction is launched between with pre-coated ApoB48 and ApoB48 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of ApoB48 in the samples. The color development is stopped and the intensity of the color is measured.

161783809

NP_062160.2

NM_019287.2

Q7TMA5

536,024 Da

Cell Surface Interactions At The Vascular Wall Pathway (573932); Chylomicron-mediated Lipid Transport Pathway (573955); Fat Digestion And Absorption Pathway (194376); Fat Digestion And Absorption Pathway (194324); Hemostasis Pathway (573899); LDL-mediated Lipid Transport Pathway (573957); Lipid Digestion, Mobilization, And Transport Pathway (573951); Lipoprotein Metabolism Pathway (573954); Metabolism Pathway (573935); Metabolism Of Lipids And Lipoproteins Pathway (573950)

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-L and apoB-H. Unlike the apoB-48 and apoB-100 structural equivalents in human, which are synthesized exclusively in the gut and liver, respectively, the rat apoBL isoform is also found in rat liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-L protein is produced after RNA editing of the apoB-H transcript at residue 2180 (CAA- UAA), resulting in the creation of a stop codon, and early translation termination. [provided by RefSeq, Jul 2008]

APOB: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. Defects in APOB are a cause of familial hypobetalipoproteinemia type 1 (FHBL1). A disorder characterized by highly reduced plasma concentrations of low density lipoproteins, and dietary fat malabsorption. Clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia. Defects in APOB are a cause of familial ligand-defective apolipoprotein B-100 (FDB). FDB is a dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors. Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia. Protein type: Secreted, signal peptide; Carrier; Secreted. Cellular Component: Golgi apparatus; extracellular space; chylomicron; cell soma; intracellular membrane-bound organelle; endoplasmic reticulum; cytoplasm; plasma membrane; vesicle membrane; actin cytoskeleton. Molecular Function: heparin binding; lipid transporter activity; protein binding; low-density lipoprotein receptor binding; cholesterol transporter activity; phospholipid binding; lipid binding. Biological Process: lipoprotein catabolic process; response to lipopolysaccharide; response to carbohydrate stimulus; post-embryonic development; response to organic substance; response to selenium ion; triacylglycerol catabolic process; nervous system development; cholesterol metabolic process; cholesterol transport; in utero embryonic development; regulation of cholesterol biosynthetic process; response to virus; cholesterol efflux; lipoprotein metabolic process; lipid transport; sperm motility; cholesterol homeostasis; fertilization; lipoprotein transport; lipoprotein biosynthetic process; artery morphogenesis; spermatogenesis; lipid metabolic process; triacylglycerol mobilization; lipid catabolic process

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800