Human Deoxyribonuclease gamma, DNASE1L3 ELISA Kit | MyBioSource MBS925159 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Deoxyribonuclease gamma, DNASE1L3 ELISA Kit

catalog :

MBS925159

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS925159

products type :

ELISA Kit

products full name :

Human Deoxyribonuclease gamma, DNASE1L3 ELISA Kit

products short name :

deoxyribonuclease I-like 3

products name syn :

Human Deoxyribonuclease gamma (DNASE1L3) ELISA kit; DHP2; DNAS1L3; LSD; DNase I homolog protein 2; DNase gamma; LS-DNase; Liver and spleen DNase; deoxyribonuclease I-like III; deoxyribonuclease gamma; deoxyribonuclease I-like 3

other names :

deoxyribonuclease gamma isoform 2; Deoxyribonuclease gamma; deoxyribonuclease gamma; LS-DNase; DNase gamma; DNase I-like 3; Liver and spleen DNase; DNase I homolog protein 2; DNase I homolog protein DHP2; deoxyribonuclease I-like III; deoxyribonuclease I-like 3; DNase I homolog protein DHP2; Deoxyribonuclease I-like 3; DNase I-like 3; Liver and spleen DNase; LS-DNase; LSD

products gene name :

DNASE1L3

other gene names :

DNASE1L3; DNASE1L3; LSD; DHP2; SLEB16; DNAS1L3; DHP2; DNAS1L3; DNase gamma; DNase I-like 3; LS-DNase; LSD

uniprot entry name :

DNSL3_HUMAN

reactivity :

Human

sequence length :

275

specificity :

This assay has high sensitivity and excellent specificity for detection of Human DNASE1L3. No significant cross-reactivity or interference between Human DNASE1L3 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 23.44 pg/ml-1500 pg/ml. Sensitivity: 5.86 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Apoptosis/Autophagy. Protein Biological Process 3: Apoptosis

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DNASE1L3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DNASE1L3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DNASE1L3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DNASE1L3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

375151549

ncbi acc num :

NP_001243489.1

ncbi gb acc num :

NM_001256560.1

uniprot acc num :

Q13609

ncbi mol weight :

35,504 Da

ncbi summary :

This gene encodes a member of the deoxyribonuclease I family. The encoded protein hydrolyzes DNA, is not inhibited by actin, and mediates the breakdown of DNA during apoptosis. Mutations in this gene are a cause of systemic lupus erythematosus-16. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]

uniprot summary :

DNASE1L3: Has DNA hydrolytic activity. Does not bind to actin. Cleaves chromatin DNA to nucleosomal units. Defects in DNASE1L3 are the cause of systemic lupus erythematosus type 16 (SLEB16). A rare autosomal recessive form of systemic lupus erythematosus with childhood onset, characterized by high frequency of anti-neutrophil cytoplasmic antibodies and lupus nephritis. Systemic lupus erythematosus is a chronic, relapsing, inflammatory, and often febrile multisystemic disorder of connective tissue, characterized principally by involvement of the skin, joints, kidneys and serosal membranes. It is of unknown etiology, but is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The disease is marked by a wide range of system dysfunctions, an elevated erythrocyte sedimentation rate, and the formation of LE cells in the blood or bone marrow. Belongs to the DNase I family. Protein type: Secreted, signal peptide; EC 3.1.21.-; Deoxyribonuclease; Secreted; Apoptosis; Calcium-binding. Chromosomal Location of Human Ortholog: 3p14.3. Cellular Component: nucleus. Molecular Function: endodeoxyribonuclease activity, producing 5 -phosphomonoesters; DNA binding; deoxyribonuclease activity; calcium ion binding; endodeoxyribonuclease activity. Biological Process: DNA fragmentation during apoptosis; DNA metabolic process. Disease: Systemic Lupus Erythematosus 16

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage