ELISA/assay

Human Protein FAM20A, FAM20A ELISA Kit

MBS924910

48-Strip-Wells

510 USD

ELISA Kit

Human Protein FAM20A, FAM20A ELISA Kit

family with sequence similarity 20, member A

Human Protein FAM20A (FAM20A) ELISA kit; DKFZp434F2322; FP2747; ; family with sequence similarity 20; member A

protein FAM20A isoform b; Protein FAM20A; protein FAM20A; family with sequence similarity 20, member A

FAM20A

FAM20A; FAM20A; AI1G; AIGFS; FP2747; UNQ9388/PRO34279

FA20A_HUMAN

Human

541

This assay has high sensitivity and excellent specificity for detection of human FAM20A. No significant cross-reactivity or interference between human FAM20A and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of human FAM20A is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FAM20A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FAM20A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FAM20A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FAM20A bound in the initial step. The color development is stopped and the intensity of the color is measured.

344198227

NP_001230675.1

NM_001243746.1

Q96MK3

61,417 Da

This locus encodes a protein that is likely secreted and may function in hematopoiesis. A mutation at this locus has been associated with amelogenesis imperfecta and gingival hyperplasia syndrome. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Aug 2011]

FAM20A: Defects in FAM20A are the cause of amelogenesis imperfecta and gingival fibromatosis syndrome (AIGFS). An autosomal recessive condition characterized by mild gingival fibromatosis and dental anomalies, including hypoplastic amelogenesis imperfecta, intrapulpal calcifications, delay of tooth eruption, hypodontia/oligodontia, pericoronal radiolucencies, and unerupted teeth. Belongs to the FAM20 family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 17q24.2. Cellular Component: Golgi apparatus; cell. Biological Process: calcium ion homeostasis. Disease: Amelogenesis Imperfecta, Type Ig

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800