ELISA/assay

Bovine Major prion protein, PRNP ELISA Kit

MBS924608

48-Strip-Wells

565 USD

ELISA Kit

Bovine Major prion protein, PRNP ELISA Kit

prion protein

Bovine Major prion protein (PRNP) ELISA kit; ASCR; CD230; CJD; GSS; MGC26679; PRIP; PrP; PrP27-30; PrP33-35C; PrPc; prion; CD230 antigen; major prion protein; p27-30; prion protein PrP; prion-related protein; prion protein

major prion protein preproprotein; Major prion protein; alternative prion protein; major prion protein; prion protein PrP; prion protein variant a; prion protein variant b; prion protein precursor PrP; major scrapie-associated fibril protein 1; prion protein (p27-30) (Creutzfeldt-Jakob disease, Gerstmann-Strausler-Scheinker syndrome, fatal familial insomnia); prion protein<; Major scrapie-associated fibril protein 1; CD_antigen: CD230

PRNP

PRNP; PRNP; PrP; AltPrP; PRP; PrP

PRIO_BOVIN

Bovine

264

This assay has high sensitivity and excellent specificity for detection of Bovine PRNP. No significant cross-reactivity or interference between Bovine PRNP and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml-10 ng/ml. Sensitivity: 0.039 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PRNP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRNP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRNP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRNP bound in the initial step. The color development is stopped and the intensity of the color is measured.

410110920

NP_001258555.1

NM_001271626.1

P10279

28,614 Da

Axon Guidance Pathway (581959); Developmental Biology Pathway (581958); NCAM Signaling For Neurite Out-growth Pathway (581968); NCAM1 Interactions Pathway (581969); Prion Diseases Pathway (101126); Prion Diseases Pathway (100065)

Function: May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu2+ or ZN2+ for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150