catalog number :
MBS924540
products type :
ELISA Kit
products full name :
Mouse Apolipoprotein A-I-binding protein, APOA1BP ELISA Kit
products short name :
apolipoprotein A-I binding protein
products name syn :
Mouse Apolipoprotein A-I-binding protein (APOA1BP) ELISA kit; RP11-284F21.3; AIBP; MGC119143; MGC119144; MGC119145; YJEFN1; apoA-I binding protein; apolipoprotein A-I binding protein
other names :
NAD(P)H-hydrate epimerase; NAD(P)H-hydrate epimerase; NAD(P)H-hydrate epimerase; NAD(P)H-hydrate epimerase; NAD(P)HX epimerase; apoA-I binding protein; apolipoprotein A-I-binding protein; apolipoprotein A-I interacting protein; apolipoprotein A-I binding protein; Apolipoprotein A-I-binding protein; AI-BP; NAD(P)HX epimerase
products gene name :
APOA1BP
other gene names :
Apoa1bp; Apoa1bp; AIBP; AI-BP; ESTM37; Apoa1ip; AA087124; Aibp; AI-BP
uniprot entry name :
NNRE_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse APOA1BP. No significant cross-reactivity or interference between mouse APOA1BP and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 9.375 pg/ml -600 pg/ml. Sensitivity: The minimum detectable dose of mouse APOA1BP is typically less than 2.34 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for APOA1BP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any APOA1BP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for APOA1BP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of APOA1BP bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_659146.1
ncbi gb acc num :
NM_144897.3
ncbi mol weight :
30,973 Da
uniprot summary :
APOA1BP: Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S- specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX. Belongs to the nnrE/AIBP family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 5.1.99.6; Mitochondrial. Cellular Component: nucleoplasm; extracellular space; intracellular membrane-bound organelle; mitochondrion; cytoplasm; extracellular region; cilium. Molecular Function: protein homodimerization activity; metal ion binding; nucleotide binding; isomerase activity. Biological Process: NADH metabolic process; NADP metabolic process; protein homotetramerization
size4 :
10x96-Strip-Wells