Human Translationally-controlled tumor protein, TPT1 ELISA Kit | MyBioSource MBS924430 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Translationally-controlled tumor protein, TPT1 ELISA Kit

catalog :

MBS924430

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS924430

products type :

ELISA Kit

products full name :

Human Translationally-controlled tumor protein, TPT1 ELISA Kit

products short name :

tumor protein, translationally-controlled 1

products name syn :

Human Translationally-controlled tumor protein (TPT1) ELISA kit; FLJ27337; HRF; TCTP; p02; fortilin; histamine-releasing factor; tumor protein; translationally-controlled 1

other names :

translationally-controlled tumor protein isoform 3; Translationally-controlled tumor protein; translationally-controlled tumor protein; fortilin; histamine-releasing factor; tumor protein, translationally-controlled 1; Fortilin; Histamine-releasing factor; HRF; p23

products gene name :

TPT1

other gene names :

TPT1; TPT1; HRF; p02; p23; TCTP; TCTP; HRF

uniprot entry name :

TCTP_HUMAN

reactivity :

Human

sequence length :

172

specificity :

This assay has high sensitivity and excellent specificity for detection of Human TPT1. No significant cross-reactivity or interference between Human TPT1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 12.5 pg/ml-800 pg/ml. Sensitivity: 3.12 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TPT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TPT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPT1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

555943820

ncbi acc num :

NP_001273202.1

ncbi gb acc num :

NM_001286273.1

uniprot acc num :

P13693

ncbi mol weight :

19,595 Da

ncbi pathways :

PLK1 Signaling Events Pathway (138007)

uniprot summary :

TPT1: a novel heat shock protein with antiapoptotic and chaperone-like activity. Its levels are highly regulated in response to various cellular stimuli and stresses. Downregulated in response to proapoptotic treatments that induce Ca(++) stress, in a PKR-dependent manner. Colocalizes with CHFR to the mitotic spindle. Appears to be involved in calcium binding and microtubule stabilization. Its exosomal secretion is induced following DNA damage. Interacts with MCL1 and STEAP3, a p53-inducible transmembrane protein that facilitates the exosomal secretion of TPT1. Secreted TPT1 acts as a histamine-releasing factor, participates in inflammatory responses by inducing the release of histamine, and may play a role in tumor reversion. May play a direct role in placental calcium transport. Phosphorylation by Plk may decrease its microtubule-stabilizing activity and promote microtubule dynamics following metaphase. Critical for peripheral T cell maintenance and TCR-mediated cell proliferation. Protein type: Calcium-binding; Microtubule-binding. Chromosomal Location of Human Ortholog: 13q14. Cellular Component: multivesicular body; extracellular space; tubulin complex; cytoplasm; nucleus. Molecular Function: protein binding; calcium ion binding. Biological Process: cellular calcium ion homeostasis; regulation of apoptosis; response to virus; calcium ion transport; negative regulation of apoptosis

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage