Human Bcl-2-related protein A1, BCL2A1 ELISA Kit | MyBioSource MBS924274 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Bcl-2-related protein A1, BCL2A1 ELISA Kit

catalog :

MBS924274

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS924274

products type :

ELISA Kit

products full name :

Human Bcl-2-related protein A1, BCL2A1 ELISA Kit

products short name :

BCL2-related protein A1

products name syn :

Human Bcl-2-related protein A1 (BCL2A1) ELISA kit; ACC-1; ACC-2; BCL2L5; BFL1; GRS; HBPA1; hematopoietic BCL2-related protein A1; BCL2-related protein A1

other names :

bcl-2-related protein A1 isoform 2; Bcl-2-related protein A1; bcl-2-related protein A1; bcl2-L-5; protein BFL-1; bcl-2-like protein 5; hematopoietic BCL2-related protein A1; hemopoietic-specific early response protein; BCL2-related protein A1; Bcl-2-like protein 5; Bcl2-L-5; Hemopoietic-specific early response protein; Protein BFL-1; Protein GRS

products gene name :

BCL2A1

other gene names :

BCL2A1; BCL2A1; GRS; BFL1; ACC-1; ACC-2; HBPA1; BCL2L5; BCL2L5; BFL1; GRS; HBPA1; Bcl2-L-5

uniprot entry name :

B2LA1_HUMAN

reactivity :

Human

sequence length :

175

specificity :

This assay has high sensitivity and excellent specificity for detection of Human BCL2A1. No significant cross-reactivity or interference between Human BCL2A1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml-2000 pg/ml. Sensitivity: 7.81 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Apoptosis/Autophagy. Protein Biological Process 3: Apoptosis

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BCL2A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BCL2A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BCL2A1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BCL2A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

168480072

ncbi acc num :

NP_001108207.1

ncbi gb acc num :

NM_001114735.1

uniprot acc num :

Q16548

ncbi mol weight :

20,132 Da

ncbi pathways :

BCR Signaling Pathway (138058); Direct P53 Effectors Pathway (137939); NF-kappa B Signaling Pathway (634527); Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987)

ncbi summary :

This gene encodes a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators that are involved in a wide variety of cellular activities such as embryonic development, homeostasis and tumorigenesis. The protein encoded by this gene is able to reduce the release of pro-apoptotic cytochrome c from mitochondria and block caspase activation. This gene is a direct transcription target of NF-kappa B in response to inflammatory mediators, and is up-regulated by different extracellular signals, such as granulocyte-macrophage colony-stimulating factor (GM-CSF), CD40, phorbol ester and inflammatory cytokine TNF and IL-1, which suggests a cytoprotective function that is essential for lymphocyte activation as well as cell survival. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

BCL2A1: Retards apoptosis induced by IL-3 deprivation. May function in the response of hemopoietic cells to external signals and in maintaining endothelial survival during infection. Interacts directly with BAK1, BID, BMF and BBC3. Interacts directly with BCL2L11/BIM. Interacts with BAX isoform Sigma. Interacts directly with PMAIP1. By phorbol ester and inflammatory cytokines, such as TNF or IL1B/interleukin-1 beta, but not by growth factors. Seems to be restricted to the hematopoietic compartment. Expressed in peripheral blood, spleen, and bone marrow, at moderate levels in lung, small intestine and testis, at a minimal levels in other tissues. Also found in vascular smooth muscle cells and hematopoietic malignancies. Belongs to the Bcl-2 family. 2 isoforms of the human protein are produced by alternative splicing. Chromosomal Location of Human Ortholog: 15q24.3. Cellular Component: mitochondrial outer membrane. Molecular Function: BH domain binding; protein binding; protein homodimerization activity; protein heterodimerization activity. Biological Process: DNA damage response, signal transduction resulting in induction of apoptosis; negative regulation of apoptosis

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage