ELISA/assay

Human Inositol 1, 4, 5-trisphosphate receptor type 1, ITPR1 ELISA Kit

MBS924170

48-Strip-Wells

510 USD

ELISA Kit

Human Inositol 1, 4, 5-trisphosphate receptor type 1, ITPR1 ELISA Kit

inositol 1, 4, 5-triphosphate receptor, type 1

Human Inositol 1; 4; 5-trisphosphate receptor type 1 (ITPR1) ELISA kit; DKFZp313E1334; DKFZp313N1434; INSP3R1; IP3R; IP3R1; SCA15; SCA16; IP3 receptor; type 1 InsP3 receptor; inositol 1; 4; 5-triphosphate receptor; type 1

inositol 1,4,5-trisphosphate receptor type 1 isoform 1; Inositol 1,4,5-trisphosphate receptor type 1; inositol 1,4,5-trisphosphate receptor type 1; IP3R 1; IP3 receptor; type 1 InsP3 receptor; inositol 1,4,5-triphosphate receptor, type 1; protein phosphatase 1, regulatory subunit 94; type 1 inositol 1,4,5-trisphosphate receptor; inositol 1,4,5-trisphosphate receptor, type 1; IP3 receptor isoform 1; IP3R 1; InsP3R1; Type 1 inositol 1,4,5-trisphosphate receptor; Type 1 InsP3 receptor

ITPR1

ITPR1; ITPR1; ACV; CLA4; IP3R; IP3R1; SCA15; SCA16; SCA29; INSP3R1; PPP1R94; INSP3R1; IP3R 1; InsP3R1; Type 1 InsP3 receptor

ITPR1_HUMAN

Human

2,758

This assay has high sensitivity and excellent specificity for detection of human ITPR1. No significant cross-reactivity or interference between human ITPR1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 6.25 pg/ml -400 pg/ml. Sensitivity: The minimum detectable dose of human ITPR1 is typically less than 1.56 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ITPR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ITPR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ITPR1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ITPR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

269954690

NP_001093422.2

NM_001099952.2

Q14643

313,929 Da

Adaptive Immune System Pathway (366160); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Antigen Activates B Cell Receptor Leading To Generation Of Second Messengers Pathway (576249); B Cell Receptor Signaling Pathway (198909); Calcium Regulation In The Cardiac Cell Pathway (198906); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Cholinergic Synapse Pathway (217716); DAG And IP3 Signaling Pathway (477115)

This gene encodes an intracellular receptor for inositol 1,4,5-trisphosphate. Upon stimulation by inositol 1,4,5-trisphosphate, this receptor mediates calcium release from the endoplasmic reticulum. Mutations in this gene cause spinocerebellar ataxia type 15, a disease associated with an heterogeneous group of cerebellar disorders. Multiple transcript variants have been identified for this gene. [provided by RefSeq, Nov 2009]

IP3R1: a multi-pass endoplasmic reticulum membrane receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium. Plays a role in ER stress-induced apoptosis. Cytoplasmic calcium released from the ER triggers apoptosis by the activation of CaMKII, eventually leading to the activation of downstream apoptosis pathways. Part of a cGMP kinase signaling complex that includes alpha-actin, calponin H1, phospholamban, PKG1 and IP3R1. Widely expressed. The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region. Phosphorylation by PKA prevents the ligand-induced opening of the calcium channels. Belongs to the ryanodine-inositol 1,4,5-triphosphate receptor ca(2+) channel (rir-cac) family. Eight alternatively spliced isoforms have been described. Protein type: Membrane protein, integral; Channel, calcium; Membrane protein, multi-pass; Channel, ligand-gated. Chromosomal Location of Human Ortholog: 3p26.1. Cellular Component: endoplasmic reticulum membrane; membrane; sarcoplasmic reticulum; endoplasmic reticulum; postsynaptic density; integral to membrane; nucleolus; nuclear inner membrane; platelet dense granule membrane; calcineurin complex. Molecular Function: protein binding; calcium ion transmembrane transporter activity; calcium channel inhibitor activity; intracellular ligand-gated calcium channel activity; phosphoinositide binding; inositol 1,4,5-triphosphate-sensitive calcium-release channel activity. Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of calcium-mediated signaling; platelet activation; inositol phosphate-mediated signaling; voluntary musculoskeletal movement; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; signal transduction; post-embryonic development; endoplasmic reticulum calcium ion homeostasis; phospholipase C activation; release of sequestered calcium ion into cytosol; calcium ion transport; response to hypoxia; energy reserve metabolic process; innate immune response; blood coagulation; vascular endothelial growth factor receptor signaling pathway; regulation of insulin secretion

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800