ELISA/assay

Human NACHT, LRR and PYD domains-containing protein 1, NLRP1 ELISA Kit

MBS924094

48-Strip-Wells

510 USD

ELISA Kit

Human NACHT, LRR and PYD domains-containing protein 1, NLRP1 ELISA Kit

NLR family, pyrin domain containing 1

Human NACHT; LRR and PYD domains-containing protein 1 (NLRP1) ELISA kit; CARD7; CLR17.1; DEFCAP; DEFCAP-L/S; DKFZp586O1822; KIAA0926; NAC; NALP1; PP1044; SLEV1; VAMAS1; NACHT; LRR and PYD containing protein 1; NACHT; leucine rich repeat and PYD (pyrin domain) containing 1; NLR family; pyrin domain containing 1

NACHT, LRR and PYD domains-containing protein 1 isoform 5; NACHT, LRR and PYD domains-containing protein 1; NACHT, LRR and PYD domains-containing protein 1; caspase recruitment domain protein 7; NACHT, LRR and PYD containing protein 1; NACHT, leucine rich repeat and PYD containing 1; caspase recruitment domain-containing protein 7; nucleotide-binding domain and caspase recruitment domain; death effector filament-forming Ced-4-like apoptosis protein; NACHT, leucine rich repeat and PYD (pyrin domain) containing 1; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 1; NLR family, pyrin domain containing 1; Caspase recruitment domain-containing protein 7; Death effector filament-forming ced-4-like apoptosis protein; Nucleotide-binding domain and caspase recruitment domain

NLRP1

NLRP1; NLRP1; NAC; CARD7; CIDED; NALP1; SLEV1; DEFCAP; PP1044; VAMAS1; CLR17.1; DEFCAP-L/S; CARD7; DEFCAP; KIAA0926; NAC; NALP1

NALP1_HUMAN

Human

1,473

This assay has high sensitivity and excellent specificity for detection of human NLRP1. No significant cross-reactivity or interference between human NLRP1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 18.75 pg/ml-1200 pg/ml. Sensitivity: 4.67 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NLRP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NLRP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NLRP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NLRP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

74271814

NP_001028225.1

NM_001033053.2

Q9C000

165,866 Da

Cellular Roles Of Anthrax Toxin Pathway (138076); Immune System Pathway (106386); Inflammasomes Pathway (366166); Innate Immune System Pathway (106387); NOD-like Receptor Signaling Pathway (122191); NOD-like Receptor Signaling Pathway (122131); Nucleotide-binding Domain, Leucine Rich Repeat Containing Receptor (NLR) Signaling Pathways (366164); The NLRP1 Inflammasome Pathway (366168)

This gene encodes a member of the Ced-4 family of apoptosis proteins. Ced-family members contain a caspase recruitment domain (CARD) and are known to be key mediators of programmed cell death. The encoded protein contains a distinct N-terminal pyrin-like motif, which is possibly involved in protein-protein interactions. This protein interacts strongly with caspase 2 and weakly with caspase 9. Overexpression of this gene was demonstrated to induce apoptosis in cells. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

NALP1: Able to form cytoplasmic structures termed death effector filaments. Enhances APAF1 and cytochrome c-dependent activation of pro-caspase-9 and consecutive apoptosis. Stimulates apoptosis through activation of caspase-3. Involved in activation of caspase-1 and caspase-5 as part of the NALP1 inflammasome complex which leads to processing and release of IL1B and IL18. Binds ATP. Interacts strongly with caspase-2, weakly with caspase-9 and with APAF1 in a cytochrome c-inducible way, leading to the formation of an apoptosome. This interaction may be ATP-dependent. Part of the NALP1 inflammasome complex which is involved in activation of caspase-1 and caspase-5, leading to processing of IL1B and IL18. The complex is activated by bacterial muramyl dipeptide which triggers ATP-binding and oligomerization of NALP1. Widely expressed. Isoform 1 and isoform 2 are expressed in peripheral blood leukocytes and chronic myelogenous leukemia cell line K-562, followed by thymus, spleen and heart. Also detected in brain, lung, placenta, small intestine, colon, kidney, liver, muscle, testis and epithelial cells. Absent from hematopoietic progenitor cells but expressed upon differentiation of cells into granulocytes and, to a lesser extent, monocytes. In peripheral blood cells, highest levels are found in T-lymphocytes, granulocytes and monocytes. Expression is significantly increased in bone marrow blast cells of some acute leukemia patients but not in solid tumors. Belongs to the NLRP family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Apoptosis. Chromosomal Location of Human Ortholog: 17p13.2. Cellular Component: intracellular; cytosol; nucleus. Molecular Function: protein domain specific binding; protein binding; enzyme binding; caspase activator activity; ATP binding. Biological Process: caspase activation; neuron apoptosis; apoptosis; response to muramyl dipeptide; defense response to bacterium; regulation of inflammatory response; innate immune response; positive regulation of interleukin-1 beta secretion. Disease: Vitiligo-associated Multiple Autoimmune Disease Susceptibility 1; Corneal Intraepithelial Dyskeratosis And Ectodermal Dysplasia

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800