catalog number :
MBS924059
products type :
ELISA Kit
products full name :
Human Platelet factor 4 variant, PF4V1 ELISA Kit
products short name :
[platelet factor 4 variant 1]
products name syn :
[Human Platelet factor 4 variant (PF4V1) ELISA kit; CXCL4L1; CXCL4V1; PF4-ALT; PF4A; SCYB4V1; platelet factor 4; variant 1 (PF4-like) ; platelet factor 4 variant 1]
other names :
[platelet factor 4 variant; Platelet factor 4 variant; platelet factor 4 variant; PF4alt; PF4var1; C-X-C motif chemokine 4; platelet factor 4, variant 1 (PF4-like); platelet factor 4 variant 1; C-X-C motif chemokine 4 variant; CXCL4L1; PF4alt; PF4var1Cleaved into the following 3 chains:Platelet factor 4 variant(4-74); Platelet factor 4 variant(5-74); Platelet factor 4 variant(6-74)]
products gene name :
[PF4V1]
other gene names :
[PF4V1; PF4V1; PF4A; CXCL4L1; CXCL4V1; PF4-ALT; SCYB4V1; CXCL4V1; SCYB4V1]
uniprot entry name :
PF4V_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human PF4V1. No significant cross-reactivity or interference between human PF4V1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml -40 ng/ml. Sensitivity: 0.156 ng /ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PF4V1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PF4V1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PF4V1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PF4V1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_002611.1
ncbi gb acc num :
NM_002620.2
ncbi mol weight :
11,553 Da
ncbi pathways :
Cell Surface Interactions At The Vascular Wall Pathway (106062); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Common Pathway (106060); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Formation Of Fibrin Clot (Clotting Cascade) Pathway (106057); Hemostasis Pathway (106028)
uniprot summary :
PF4V1: Inhibitor of angiogenesis. Inhibitor of endothelial cell chemotaxis (in vitro). Belongs to the intercrine alpha (chemokine CxC) family. Protein type: Secreted; Chemokine; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 4q12-q21. Cellular Component: extracellular space. Molecular Function: heparin binding; chemokine activity; CXCR chemokine receptor binding. Biological Process: G-protein coupled receptor protein signaling pathway; response to lipopolysaccharide; immune response; positive regulation of leukocyte chemotaxis; inflammatory response; regulation of cell proliferation
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells