ELISA/assay

Mouse Protein Wnt-7a, WNT7A ELISA Kit

MBS924046

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Mouse Protein Wnt-7a, WNT7A ELISA Kit

[wingless-type MMTV integration site family, member 7A]

[Mouse Protein Wnt-7a (WNT7A) ELISA kit; proto-oncogene Wnt7a protein; wingless-type MMTV integration site family; member 7A]

[protein Wnt-7a; Protein Wnt-7a; protein Wnt-7a; postaxial hemimelia; wingless-related MMTV integration site 7A; wingless-type MMTV integration site family, member 7A]

[WNT7A]

[Wnt7a; Wnt7a; px; tw; Wnt-7a; AI849442; Wnt-7a]

WNT7A_MOUSE

Mouse

349

This assay has high sensitivity and excellent specificity for detection of mouse WNT7A. No significant cross-reactivity or interference between mouse WNT7A and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Quantitative Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: Typically less than 0.039 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for WNT7A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT7A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT7A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT7A bound in the initial step. The color development is stopped and the intensity of the color is measured.

31543960

NP_033553.2

NM_009527.3

P24383

38,974 Da

Basal Cell Carcinoma Pathway (83306); Basal Cell Carcinoma Pathway (525); ESC Pluripotency Pathways (198374); HTLV-I Infection Pathway (373903); HTLV-I Infection Pathway (373889); Hedgehog Signaling Pathway (83260); Hedgehog Signaling Pathway (474); Melanogenesis Pathway (83289); Melanogenesis Pathway (504); Pathways In Cancer (83298)

WNT7A: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. Signaling by Wnt-7a allows sexually dimorphic development of the mullerian ducts. Defects in WNT7A are the cause of limb pelvis hypoplasia aplasia syndrome (LPHAS). A syndrome of severe deficiency of the extremities due to hypo- or aplasia of one or more long bones of one or more limbs. Pelvic manifestations include hip dislocation, hypoplastic iliac bone and aplastic pubic bones. Thoracic deformity, unusual facies and genitourinary anomalies can be present. Defects in WNT7A are a cause of Fuhrmann syndrome (FUHRS); also known as fibular aplasia or hypoplasia femoral bowing and poly- syn- and oligodactyly. Fuhrmann syndrome is a distinct limb-malformation disorder characterized also by various degrees of limb aplasia/hypoplasia and joint dysplasia. Belongs to the Wnt family. Protein type: Secreted, signal peptide; Secreted. Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; cell; extracellular region. Molecular Function: protein binding; frizzled binding; cytokine activity; receptor agonist activity; receptor binding. Biological Process: hindlimb morphogenesis; somatic stem cell maintenance; positive regulation of epithelial cell proliferation involved in wound healing; multicellular organismal development; positive regulation of transcription, DNA-dependent; Wnt receptor signaling pathway through beta-catenin; uterus development; cell-cell signaling; synapse organization and biogenesis; regulation of axon diameter; chondrocyte differentiation; somatic stem cell division; satellite cell activation; embryonic limb morphogenesis; cartilage condensation; cell fate commitment; skin morphogenesis; asymmetric protein localization; positive regulation of synaptogenesis; organ morphogenesis; response to estrogen stimulus; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; negative regulation of apoptosis; embryonic forelimb morphogenesis; limb development; neurotransmitter secretion; cell proliferation in forebrain; positive regulation of JNK cascade; palate development; regulation of axonogenesis; signal transduction; embryonic hindlimb morphogenesis; negative regulation of neurogenesis; forelimb morphogenesis; central nervous system vasculogenesis; positive regulation of cell proliferation; angiogenesis; fallopian tube development; Wnt receptor signaling pathway; embryonic axis specification; satellite cell compartment self-renewal involved in skeletal muscle regeneration; cerebellar granule cell differentiation; regulation of cell proliferation; stem cell development; dorsal/ventral pattern formation; skeletal morphogenesis; establishment of cell polarity; cartilage development; reproductive structure development

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

495

96-Strip-Wells

710

5x96-Strip-Wells

2705

10x96-Strip-Wells

5145