ELISA/assay

Human Interleukin-31 receptor subunit alpha, IL31RA ELISA Kit

MBS923904

48-Strip-Wells

510 USD

ELISA Kit

Human Interleukin-31 receptor subunit alpha, IL31RA ELISA Kit

interleukin 31 receptor A

Human Interleukin-31 receptor subunit alpha (IL31RA) ELISA kit; CRL; CRL3; GLM-R; GLMR; GPL; IL-31RA; MGC125346; PRO21384; GP130 like receptor; class I cytokine receptor; gp130-like monocyte receptor; soluble type I cytokine receptor CRL3; interleukin 31 receptor A

interleukin-31 receptor subunit alpha isoform 2; Interleukin-31 receptor subunit alpha; interleukin-31 receptor subunit alpha; zcytoR17; IL-31R subunit alpha; cytokine receptor-like 3; class I cytokine receptor; IL-31 receptor subunit alpha; gp130-like monocyte receptor; soluble type I cytokine receptor CRL3; interleukin 31 receptor A; Cytokine receptor-like 3; GLM-R; hGLM-R; Gp130-like monocyte receptor; Gp130-like receptor; ZcytoR17

IL31RA

IL31RA; IL31RA; CRL; GPL; CRL3; GLMR; GLM-R; PLCA2; hGLM-R; IL-31RA; PRO21384; CRL3; GPL; UNQ6368/PRO21073/PRO21384; IL-31 receptor subunit alpha; IL-31R subunit alpha; IL-31R-alpha; IL-31RA; hGLM-R; Gp130-like receptor

IL31R_HUMAN

Human

732

This assay has high sensitivity and excellent specificity for detection of Human IL31RA. No significant cross-reactivity or interference between Human IL31RA and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 6.25 pg/ml-400 pg/ml. Sensitivity: 1.56 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Immunity. Protein Biological Process 3: Immunity

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL31RA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL31RA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL31RA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL31RA bound in the initial step. The color development is stopped and the intensity of the color is measured.

336391156

NP_001229565.1

NM_001242636.1

Q8NI17

82,954 Da

The protein encoded by this gene belongs to the type I cytokine receptor family. This receptor, with homology to gp130, is expressed on monocytes, and is involved in IL-31 signaling via activation of STAT-3 and STAT-5. It functions either as a monomer, or as part of a receptor complex with oncostatin M receptor (OSMR). Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jun 2011]

IL31RA: Associates with OSMR to form the interleukin-31 receptor which activates STAT3 and to a lower extent STAT1 and STAT5. May function in skin immunity. Defects in IL31RA are the cause of amyloidosis primary localized cutaneous type 2 (PLCA2). PLCA2 is primary amyloidosis characterized by localized cutaneous amyloid deposition. This condition usually presents with itching (especially on the lower legs) and visible changes of skin hyperpigmentation and thickening that may be exacerbated by chronic scratching and rubbing. Primary localized cutaneous amyloidosis is often divided into macular and lichen subtypes although many affected individuals often show both variants coexisting. Lichen amyloidosis characteristically presents as a pruritic eruption of grouped hyperkeratotic papules with a predilection for the shins, calves, ankles and dorsa of feet and thighs. Papules may coalesce to form hyperkeratotic plaques that can resemble lichen planus, lichen simplex or nodular prurigo. Macular amyloidosis is characterized by small pigmented macules that may merge to produce macular hyperpigmentation, sometimes with a reticulate or rippled pattern. In macular and lichen amyloidosis, amyloid is deposited in the papillary dermis in association with grouped colloid bodies, thought to represent degenerate basal keratinocytes. The amyloid deposits probably reflect a combination of degenerate keratin filaments, serum amyloid P component, and deposition of immunoglobulins. Belongs to the type I cytokine receptor family. Type 2 subfamily. 12 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Receptor, cytokine. Chromosomal Location of Human Ortholog: 5q11.2. Cellular Component: integral to membrane; plasma membrane. Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; cytokine binding; transcription coactivator activity; protein kinase binding. Biological Process: macrophage differentiation; monocyte differentiation; cytokine and chemokine mediated signaling pathway; positive regulation of transcription, DNA-dependent; MAPKKK cascade; defense response; JAK-STAT cascade; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of tyrosine phosphorylation of Stat5 protein; homeostatic process; positive regulation of cell proliferation; negative regulation of macrophage activation; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis. Disease: Amyloidosis, Primary Localized Cutaneous, 2

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800