Mouse Alpha-2-macroglobulin-P, A2MP ELISA Kit | MyBioSource MBS923863 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Alpha-2-macroglobulin-P, A2MP ELISA Kit

catalog :

MBS923863

quantity :

24-Strip-Wells (LIMIT 1)

price :

240 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS923863

products type :

ELISA Kit

products full name :

Mouse Alpha-2-macroglobulin-P, A2MP ELISA Kit

products short name :

[alpha-2-macroglobulin pseudogene]

products name syn :

[Mouse Alpha-2-macroglobulin-P (A2MP) ELISA kit; alpha-2-macroglobulin pseudogene]

other names :

[alpha-2-macroglobulin-P; Alpha-2-macroglobulin-P; alpha-2-macroglobulin-P; alpha-2-macroglobulin; Alpha-2-macroglobulin]

products gene name :

[A2MP]

other gene names :

[A2m; A2mp; A2mp; A2m]

uniprot entry name :

A2MP_MOUSE

reactivity :

Mouse

sequence length :

1,474

specificity :

This assay has high sensitivity and excellent specificity for detection of rat 8-OHdG. No significant cross-reactivity or interference between rat 8-OHdG and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, Plasma, Cell Culture Supernates, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.312 ng/ml-20 ng/ml. Sensitivity: 0.078 ng/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for 8-OHdG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any 8-OHdG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 8-OHdG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 8-OHdG bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

148277039

ncbi acc num :

NP_783327.2

ncbi gb acc num :

NM_175628.3

uniprot acc num :

Q6GQT1

ncbi mol weight :

164,353 Da

ncbi pathways :

Complement And Coagulation Cascades Pathway (198335); Complement And Coagulation Cascades Pathway (83270); Complement And Coagulation Cascades Pathway (484); Formation Of Fibrin Clot (Clotting Cascade) Pathway (575502); HDL-mediated Lipid Transport Pathway (574760); Hemostasis Pathway (575479); Intrinsic Pathway (575504); Lipid Digestion, Mobilization, And Transport Pathway (574755); Lipoprotein Metabolism Pathway (574758); Metabolism Pathway (574739)

uniprot summary :

A2M: Is able to inhibit all four classes of proteinases by a unique trapping mechanism. This protein has a peptide stretch, called the bait region which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family. Protein type: Secreted; Inhibitor; Secreted, signal peptide. Cellular Component: extracellular space; extracellular region. Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; enzyme binding; protease binding; protease inhibitor activity; growth factor binding; endopeptidase inhibitor activity; interleukin-1 binding; interleukin-8 binding; calcium-dependent protein binding; tumor necrosis factor binding; receptor binding. Biological Process: negative regulation of complement activation, lectin pathway; negative regulation of peptidase activity; female pregnancy; stem cell differentiation

size1 :

24-Strip-Wells (LIMIT 1)

price1 :

240 USD

size2 :

48-Strip-Wells

price2 :

495

size3 :

96-Strip-Wells

price3 :

710

size4 :

5x96-Strip-Wells

price4 :

2705

size5 :

10x96-Strip-Wells

price5 :

5145

more info or order :

MyBioSource product webpage