Mouse Glucose Regulated Protein 78, GRP78 ELISA Kit | MyBioSource MBS923855 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Glucose Regulated Protein 78, GRP78 ELISA Kit

catalog :

MBS923855

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS923855

products type :

ELISA Kit

products full name :

Mouse Glucose Regulated Protein 78, GRP78 ELISA Kit

products short name :

Glucose Regulated Protein 78 (GRP78) Elisa kit

products name syn :

Mouse Glucose Regulated Protein 78 (GRP78) Elisa kit; BIP; FLJ26106; GRP78; MIF2; Heat-shock 70kD protein-5 (glucose-regulated protein; 78kD) ; heat shock 70kD protein 5 (glucose-regulated protein; 78kD) ; heat shock 70kDa protein 5; Glucose Regulated Protein 78 (GRP78) Elisa kit

other names :

78 kDa glucose-regulated protein; 78 kDa glucose-regulated protein; 78 kDa glucose-regulated protein; GRP-78; XAP-1 antigen; heat shock 70 kDa protein 5; glucose regulated protein, 78 kDa; immunoglobulin heavy chain-binding protein; heat shock 70kD protein 5 (glucose-regulated protein, 78kD); heat shock protein 5; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP

products gene name :

GRP78

other gene names :

Hspa5; Hspa5; Bip; Sez7; mBiP; Grp78; SEZ-7; Hsce70; baffled; AL022860; AU019543; D2Wsu17e; D2Wsu141e; Grp78; GRP-78; BiP

uniprot entry name :

GRP78_MOUSE

reactivity :

Mouse

sequence length :

655

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse GRP78. No significant cross-reactivity or interference between mouse GRP78 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Quantitative Sandwich. Sensitivity: Typically less than 0.11 ng/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GRP78 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GRP78 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GRP78 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GRP78 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

254540168

ncbi acc num :

NP_001156906.1

ncbi gb acc num :

NM_001163434.1

uniprot acc num :

P20029

ncbi mol weight :

72,422 Da

ncbi pathways :

Activation Of Chaperones By ATF6-alpha Pathway (574953); Activation Of Chaperones By IRE1alpha Pathway (574954); Antigen Processing And Presentation Pathway (83271); Antigen Processing And Presentation Pathway (485); Diabetes Pathways (574950); Disease Pathway (574923); Hemostasis Pathway (575479); MAPK Signaling Pathway (198294); PERK Regulated Gene Expression Pathway (574955); Platelet Activation, Signaling And Aggregation Pathway (575489)

uniprot summary :

GRP78: a member of the HSP family of molecular chaperones required for endoplasmic reticulum integrity and stress-induced autophagy. Plays a central role in regulating the unfolded protein response (UPR), and is an obligatory component of autophagy in mammalian cells. May play an important role in cellular adaptation and oncogenic survival. One of the client proteins of GRP78 is protein double-stranded RNA-activated protein-like endoplasmic reticulum kinase (PERK). Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. Protein type: Chaperone; Heat shock protein. Cellular Component: signalosome; endoplasmic reticulum membrane; smooth endoplasmic reticulum; cell surface; focal adhesion; mitochondrion; endoplasmic reticulum; endoplasmic reticulum lumen; ER-Golgi intermediate compartment; membrane; cytoplasm; plasma membrane; integral to endoplasmic reticulum membrane; midbody; nucleus; myelin sheath. Molecular Function: protein domain specific binding; protein binding; enzyme binding; ubiquitin protein ligase binding; unfolded protein binding; nucleotide binding; ribosome binding; misfolded protein binding; glycoprotein binding; ATP binding. Biological Process: cellular response to glucose starvation; cerebellum structural organization; unfolded protein response, activation of signaling protein activity; positive regulation of embryonic development; proteolysis involved in cellular protein catabolic process; positive regulation of protein ubiquitination; cerebellar Purkinje cell layer development; negative regulation of transforming growth factor beta receptor signaling pathway; ER overload response; positive regulation of cell migration; negative regulation of apoptosis

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage