catalog number :
MBS923729
products type :
ELISA Kit
products full name :
Mouse Apolipoprotein C-II, APOC2 ELISA Kit
products short name :
[apolipoprotein C-II]
products name syn :
[Mouse Apolipoprotein C-II (APOC2) ELISA kit; MGC75082; ; apolipoprotein C-II]
other names :
[apolipoprotein C-II; Apolipoprotein C-II; apolipoprotein C-II; apo-CII; apoC-II; apolipoprotein C2; apolipoprotein C-II; Apolipoprotein C2]
products gene name :
[APOC2]
other gene names :
[Apoc2; Apoc2; Apo-CII; ApoC-II]
uniprot entry name :
APOC2_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse APOC2. No significant cross-reactivity or interference between mouse APOC2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 18.8 ng/ml -1200 ng/ml. Sensitivity: The minimum detectable dose of mouse APOC2 is typically less than 9.4 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest mouse APOC2 concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
. Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with APOC2. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for APOC2. The competitive inhibition reaction is launched between with pre-coated APOC2 and APOC2 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of APOC2 in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001264873.1
ncbi gb acc num :
NM_001277944.1
ncbi mol weight :
10,741 Da
ncbi pathways :
Chylomicron-mediated Lipid Transport Pathway (574759); HDL-mediated Lipid Transport Pathway (574760); Lipid Digestion, Mobilization, And Transport Pathway (574755); Lipoprotein Metabolism Pathway (574758); Metabolism Pathway (574739); Metabolism Of Lipids And Lipoproteins Pathway (574754); Statin Pathway (198363)
uniprot summary :
APOC2: Component of the very low density lipoprotein (VLDL) fraction in plasma, and is an activator of several triacylglycerol lipases. The association of APOC2 with plasma chylomicrons, VLDL, and HDL is reversible, a function of the secretion and catabolism of triglyceride-rich lipoproteins, and changes rapidly. Defects in APOC2 are the cause of hyperlipoproteinemia type 1B (HLPP1B). It is an autosomal recessive trait characterized by hypertriglyceridemia, xanthomas, and increased risk of pancreatitis and early atherosclerosis. Belongs to the apolipoprotein C2 family. Protein type: Secreted, signal peptide; Secreted. Cellular Component: extracellular space; chylomicron; extracellular region. Molecular Function: phospholipase binding; protein homodimerization activity; lipase inhibitor activity; enzyme activator activity; lipid binding; phospholipase activator activity. Biological Process: positive regulation of fatty acid biosynthetic process; negative regulation of cholesterol transport; cholesterol efflux; lipid transport; phospholipid efflux; transport; lipoprotein transport; negative regulation of catalytic activity; positive regulation of lipoprotein lipase activity; negative regulation of receptor-mediated endocytosis; lipid metabolic process; negative regulation of lipid metabolic process; lipid catabolic process
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells