Human Tryptase beta-2, TPSB2 ELISA Kit | MyBioSource MBS923703 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Tryptase beta-2, TPSB2 ELISA Kit

catalog :

MBS923703

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS923703

products type :

ELISA Kit

products full name :

Human Tryptase beta-2, TPSB2 ELISA Kit

products short name :

tryptase beta 2

products name syn :

Human Tryptase beta-2 (TPSB2) ELISA kit; TPS2; tryptaseB; tryptaseC; OTTHUMP00000208944; mast cell tryptase beta II; mast cell tryptase beta III; tryptase II; tryptase III; tryptase beta 2; tryptase beta II; tryptase beta III; tryptase beta 2

other names :

tryptase alpha/beta-1; Tryptase beta-2; tryptase alpha/beta-1; tryptase I; tryptase-1; tryptase-I; tryptase-III; tryptase beta 1; tryptase beta I; tryptase beta-1; tryptase alpha-1; tryptase alpha II; mast cell beta I tryptase; mast cell alpha II tryptase; tryptase alpha/beta 1; Tryptase II

products gene name :

TPSB2

other gene names :

TPSAB1; TPSB2; TPS1; TPS2; TPSB1; TPS2; Tryptase-2

uniprot entry name :

TRYB2_HUMAN

reactivity :

Human

sequence length :

275

specificity :

This assay has high sensitivity and excellent specificity for detection of human TPSB2. No significant cross-reactivity or interference between human TPSB2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 125 pg/ml -8000 pg/ml. Sensitivity: The minimum detectable dose of human TPSB2 is typically less than 31.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TPSB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TPSB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPSB2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPSB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

13775595

ncbi acc num :

NP_003285.2

ncbi gb acc num :

NM_003294.3

uniprot acc num :

P20231

ncbi mol weight :

30,515 Da

ncbi pathways :

Activation Of Matrix Metalloproteinases Pathway (576264); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262)

ncbi summary :

Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq, Jul 2008]

uniprot summary :

TPSB2: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Has an immunoprotective role during bacterial infection. Required to efficiently combat K.pneumoniae infection. Belongs to the peptidase S1 family. Tryptase subfamily. Protein type: EC 3.4.21.59; Secreted; Secreted, signal peptide; Protease. Chromosomal Location of Human Ortholog: 16p13.3. Cellular Component: extracellular region. Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity. Biological Process: proteolysis

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage