catalog number :
MBS923543
products type :
ELISA Kit
products full name :
Mouse Gastrokine-1, GKN1 ELISA Kit
products short name :
gastrokine 1
products name syn :
Mouse Gastrokine-1 (GKN1) ELISA kit; AMP18; BRICD1; CA11; FOV; MGC70354; foveolin; 18 kDa antrum mucosa protein; BRICHOS domain containing 1; gastrokine 1
other names :
gastrokine-1; Gastrokine-1; gastrokine-1; foveolin; protein CA11 homolog; 18 kDa antrum mucosa protein; gastrokine 1; 18 kDa antrum mucosa protein; AMP-18; Protein CA11 homolog
products gene name :
GKN1
other gene names :
Gkn1; Gkn1; Fov; AMP-18; BRICD1; 2200002K21Rik; Amp18; Ca11; AMP-18
uniprot entry name :
GKN1_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse GKN1. No significant cross-reactivity or interference between mouse GKN1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0. 156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of mouse GKN1 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GKN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GKN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GKN1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GKN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_079742.1
ncbi gb acc num :
NM_025466.1
ncbi mol weight :
21,887 Da
uniprot summary :
GKN1: Has mitogenic activity and may be involved in maintaining the integrity of the gastric mucosal epithelium. Belongs to the gastrokine family. Protein type: Secreted, signal peptide; Secreted. Cellular Component: extracellular region; secretory granule. Molecular Function: growth factor activity. Biological Process: positive regulation of cell division; positive regulation of cell proliferation
size4 :
10x96-Strip-Wells