Mouse Methylcytosine dioxygenase TET2, TET2 ELISA Kit | MyBioSource MBS923538 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Methylcytosine dioxygenase TET2, TET2 ELISA Kit

catalog :

MBS923538

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS923538

products type :

ELISA Kit

products full name :

Mouse Methylcytosine dioxygenase TET2, TET2 ELISA Kit

products short name :

tet oncogene family member 2

products name syn :

Mouse Methylcytosine dioxygenase TET2 (TET2) ELISA kit; FLJ20032; KIAA1546; MGC125715; OTTHUMP00000161869; tet oncogene family member 2

other names :

methylcytosine dioxygenase TET2; Methylcytosine dioxygenase TET2; methylcytosine dioxygenase TET2; tet oncogene 2; tet oncogene family member 2; probable methylcytosine dioxygenase TET2; tet methylcytosine dioxygenase 2; Protein Ayu17-449

products gene name :

TET2

other gene names :

Tet2; Tet2; Ayu17-449; mKIAA1546; E130014J05Rik; Kiaa1546

uniprot entry name :

TET2_MOUSE

reactivity :

Mouse

sequence length :

1,912

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse TET2. No significant cross-reactivity or interference between mouse TET2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 23.44 pg/ml -1500 pg/ml. Sensitivity: The minimum detectable dose of mouse TET2 is typically less than 5.86 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TET2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TET2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TET2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TET2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

157057152

ncbi acc num :

NP_001035490.2

ncbi gb acc num :

NM_001040400.2

uniprot acc num :

Q4JK59

ncbi mol weight :

212,130 Da

uniprot summary :

TET2: Catalyzes the conversion of methylcytosine (5mC) to 5- hydroxymethylcytosine (hmC). Plays an important role in myelopoiesis. The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. TET2 is frequently mutated in myeloproliferative disorders (MPD). These constitute a heterogeneous group of disorders, also known as myeloproliferative diseases or myeloproliferative neoplasms (MPN), characterized by cellular proliferation of one or more hematologic cell lines in the peripheral blood, distinct from acute leukemia. Included diseases are: essential thrombocythemia, polycythemia vera, primary myelofibrosis (chronic idiopathic myelofibrosis). Bone marrow samples from patients display uniformly low levels of hmC in genomic DNA compared to bone marrow samples from healthy controls as well as hypomethylation relative to controls at the majority of differentially methylated CpG sites. Defects in TET2 are a cause of polycythemia vera (PV). A myeloproliferative disorder characterized by abnormal proliferation of all hematopoietic bone marrow elements, erythroid hyperplasia, an absolute increase in total blood volume, but also by myeloid leukocytosis, thrombocytosis and splenomegaly. TET2 is frequently mutated in systemic mastocytosis; also known as systemic mast cell disease. A condition with features in common with myeloproliferative diseases. It is a clonal disorder of the mast cell and its precursor cells. The clinical symptoms and signs of systemic mastocytosis are due to accumulation of clonally derived mast cells in different tissues, including bone marrow, skin, the gastrointestinal tract, the liver, and the spleen. Defects in TET2 are a cause of myelodysplastic syndrome (MDS). A heterogeneous group of closely related clonal hematopoietic disorders. All are characterized by a hypercellular or hypocellular bone marrow with impaired morphology and maturation, dysplasia of the myeloid, megakaryocytic and/or erythroid lineages, and peripheral blood cytopenias resulting from ineffective blood cell production. Included diseases are: refractory anemia (RA), refractory anemia with ringed sideroblasts (RARS), refractory anemia with excess blasts (RAEB), refractory cytopenia with multilineage dysplasia and ringed sideroblasts (RCMD-RS). Chronic myelomonocytic leukemia (CMML) is a myelodysplastic/myeloproliferative disease. Myelodysplastic syndromes are considered a premalignant condition in a subgroup of patients that often progresses to acute myeloid leukemia (AML). Bone marrow samples from patients display uniformly low levels of hmC in genomic DNA compared to bone marrow samples from healthy controls as well as hypomethylation relative to controls at the majority of differentially methylated CpG sites. Belongs to the TET family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Tumor suppressor; EC 1.14.11.n2; Oxidoreductase. Cellular Component: nucleus. Molecular Function: DNA binding; zinc ion binding; dioxygenase activity; ferrous iron binding; metal ion binding; oxidoreductase activity. Biological Process: spleen development; protein amino acid O-linked glycosylation; hemoglobin metabolic process; homeostasis of number of cells; 5-methylcytosine metabolic process; chromatin modification; cell cycle; post-embryonic development; cytosine metabolic process; myeloid progenitor cell differentiation; 5-methylcytosine catabolic process; positive regulation of transcription from RNA polymerase II promoter; hemopoiesis; myeloid cell differentiation; kidney development

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage