Rat Gamma-glutamyltranspeptidase 1, GGT1 ELISA Kit | MyBioSource MBS923419 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Gamma-glutamyltranspeptidase 1, GGT1 ELISA Kit

catalog :

MBS923419

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS923419

products type :

ELISA Kit

products full name :

Rat Gamma-glutamyltranspeptidase 1, GGT1 ELISA Kit

products short name :

gamma-glutamyltransferase 1

products name syn :

Rat Gamma-glutamyltranspeptidase 1 (GGT1) ELISA kit; CD224; D22S672; D22S732; GGT; GTG; MGC96892; MGC96904; MGC96963; OTTHUMP00000028921; OTTHUMP00000159078; gamma-glutamyl transpeptidase; glutamyl transpeptidase; gamma-glutamyltransferase 1

other names :

gamma-glutamyltranspeptidase 1; Gamma-glutamyltranspeptidase 1; gamma-glutamyltranspeptidase 1; GGT 1; glutathione hydrolase 1; leukotriene-C4 hydrolase; gamma glutamyl-transferase 1; gamma-glutamyl transpeptidase; gamma-glutamyltransferase 1; Gamma-glutamyltransferase 1; Glutathione hydrolase 1 (EC:3.4.19.13); Leukotriene-C4 hydrolase (EC:3.4.19.14); CD_antigen: CD224Cleaved into the following 2 chains:Gamma-glutamyltranspeptidase 1 heavy chain; Gamma-glutamyltranspeptidase 1 light chain

products gene name :

GGT1

other gene names :

Ggt1; Ggt1; Ggt; Ggtp; GGLUT; Ggt; GGT 1

uniprot entry name :

GGT1_RAT

reactivity :

Rat

sequence length :

568

specificity :

This assay has high sensitivity and excellent specificity for detection of rat GGT1. No significant cross-reactivity or interference between rat GGT1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 4.7 mIU/ml-300 mIU/ml. Sensitivity: 1.2 mIU/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GGT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GGT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GGT1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GGT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

145280505

ncbi acc num :

NP_446292.2

ncbi gb acc num :

NM_053840.2

uniprot acc num :

P07314

ncbi mol weight :

61,610 Da

ncbi pathways :

Arachidonic Acid Metabolism Pathway (83383); Arachidonic Acid Metabolism Pathway (366); Cyanoamino Acid Metabolism Pathway (83362); Cyanoamino Acid Metabolism Pathway (339); Eicosanoid Synthesis Pathway (198497); Glutathione Metabolism Pathway (83365); Glutathione Metabolism Pathway (343); Taurine And Hypotaurine Metabolism Pathway (83359); Taurine And Hypotaurine Metabolism Pathway (336)

ncbi summary :

required in the glutathione metabolism [RGD, Feb 2006]

uniprot summary :

GGT1: Initiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracellular GSH level. It is part of the cell antioxidant defense mechanism. Catalyzes the transfer of the glutamyl moiety of glutathione to amino acids and dipeptide acceptors. Alternatively, glutathione can be hydrolyzed to give Cys-Gly and gamma glutamate. Isoform 3 seems to be inactive. Defects in GGT1 are a cause of glutathionuria (GLUTH); also known as gamma-glutamyltranspeptidase deficiency. It is an autosomal recessive disease. Belongs to the gamma-glutamyltransferase family. 3 isoforms of the human protein are produced by alternative promoter. Protein type: Membrane protein, integral; Other Amino Acids Metabolism - cyanoamino acid; Lipid Metabolism - arachidonic acid; Transferase; EC 3.4.19.14; EC 3.4.19.13; EC 2.3.2.2; Other Amino Acids Metabolism - taurine and hypotaurine; Other Amino Acids Metabolism - selenoamino acid; Other Amino Acids Metabolism - glutathione. Cellular Component: extracellular space; integral to plasma membrane; plasma membrane. Molecular Function: gamma-glutamyltransferase activity. Biological Process: peptide modification; glutamate metabolic process; regulation of immune system process; cysteine biosynthetic process; response to lipopolysaccharide; proteolysis; response to estradiol stimulus; leukotriene biosynthetic process; regulation of inflammatory response; glutathione catabolic process; glutathione biosynthetic process; spermatogenesis; zymogen activation; aging

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage