Rat Glia-derived nexin, SERPINE2 ELISA Kit | MyBioSource MBS923401 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Glia-derived nexin, SERPINE2 ELISA Kit

catalog :

MBS923401

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS923401

products type :

ELISA Kit

products full name :

Rat Glia-derived nexin, SERPINE2 ELISA Kit

products short name :

serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1) , member 2

products name syn :

Rat Glia-derived nexin (SERPINE2) ELISA kit; GDN; PI7; PN1; PNI; OTTHUMP00000203766; glial-derived neurite promoting factor; glial-derived nexin 1; plasminogen activator inhibitor type 1; member 2; protease inhibitor 7 (protease nexin I) ; serine (o; serpin peptidase inhibitor; clade E (nexin; plasminogen activator inhibitor type 1); member 2

other names :

glia-derived nexin; Glia-derived nexin; glia-derived nexin; GDN; serpin E2; protease nexin 1; protease nexin I; protease nexin PN-1; peptidase inhibitor 7; serine protease inhibitor 4; Glia-derived nexin/protease nexin I; plasminogen activator inhibitor type 1; serpin peptidase inhibitor, clade E, member 2; serine (or cysteine) peptidase inhibitor, clade E, member 2; serine (or cysteine) proteinase inhibitor, clade E, member 2; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2; Peptidase inhibitor 7; PI-7; Protease nexin 1; PN-1; Protease nexin I; Serpin E2

products gene name :

SERPINE2

other gene names :

Serpine2; Serpine2; PI-7; Pn-1; Spin4; Gdnpn1; Pi7; Pn1; GDN; PI-7; PN-1

uniprot entry name :

GDN_RAT

reactivity :

Rat

sequence length :

397

specificity :

This assay has high sensitivity and excellent specificity for detection of rat SERPINE2. No significant cross-reactivity or interference between rat SERPINE2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: The minimum detectable dose of rat SERPINE2 is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SERPINE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SERPINE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINE2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINE2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

158138559

ncbi acc num :

NP_062070.1

ncbi gb acc num :

NM_019197.1

uniprot acc num :

P07092

ncbi mol weight :

44,063 Da

ncbi summary :

displays neurite promoting activity and serine protease inhibitory activity [RGD, Feb 2006]

uniprot summary :

SERPINE2: Serine protease inhibitor with activity toward thrombin, trypsin, and urokinase. Promotes neurite extension by inhibiting thrombin. Binds heparin. Belongs to the serpin family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Inhibitor; Secreted, signal peptide. Cellular Component: extracellular matrix; extracellular space; extrinsic to external side of plasma membrane; membrane; cell soma; cell; extracellular region; cytosol; neuromuscular junction; vesicle. Molecular Function: serine-type endopeptidase inhibitor activity; heparin binding; glycosaminoglycan binding; receptor binding. Biological Process: negative regulation of proteolysis; regulation of synaptic transmission, glutamatergic; secretory granule organization and biogenesis; cerebellar granular layer morphogenesis; negative regulation of peptidase activity; negative regulation of blood coagulation; detection of mechanical stimulus involved in sensory perception; mating plug formation; negative regulation of cell proliferation; response to radiation; negative regulation of smoothened signaling pathway; response to wounding; negative regulation of cell growth; blood coagulation; negative regulation of protein catabolic process; regulation of timing of cell differentiation; embryo implantation; negative regulation of phosphoinositide 3-kinase cascade; secretion by cell; positive regulation of astrocyte differentiation

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage