ELISA/assay

Human Urocortin-2, UCN2 ELISA Kit

MBS923385

48-Strip-Wells

510 USD

ELISA Kit

Human Urocortin-2, UCN2 ELISA Kit

urocortin 2

Human Urocortin-2 (UCN2) ELISA kit; SRP; UCN-II; UCNI; UR; URP; stresscopin-related peptide; urocortin II; urocortin-related peptide; urocortin 2

urocortin-2 preproprotein; Urocortin-2; urocortin-2; ucn II; urocortin II; prepro-urocortin 2; urocortin-related peptide; stresscopin-related peptide; urocortin 2; Stresscopin-related peptide; Urocortin II; Ucn II; Urocortin-related peptide

UCN2

UCN2; UCN2; UR; SRP; URP; UCNI; UCN-II; SRP; URP; Ucn II

UCN2_HUMAN

Human

112

This assay has high sensitivity and excellent specificity for detection of Human UCN2. No significant cross-reactivity or interference between Human UCN2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, cell culture supernates. Assay Type: Sandwich. Detection Range: 6.25 pg/ml-400 pg/ml. Sensitivity: 1.56 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for UCN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any UCN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UCN2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UCN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

15082240

NP_149976.1

NM_033199.3

Q96RP3

12,146 Da

This gene is a member of the sauvagine/corticotropin-releasing factor/urotensin I family. It is structurally related to the corticotropin-releasing factor (CRF) gene and the encoded product is an endogenous ligand for CRF type 2 receptors. In the brain it may be responsible for the effects of stress on appetite. In spite of the gene family name similarity, the product of this gene has no sequence similarity to urotensin II. [provided by RefSeq, Jul 2008]

UCN2: Suppresses food intake, delays gastric emptying and decreases heat-induced edema. Might represent an endogenous ligand for maintaining homeostasis after stress. Belongs to the sauvagine/corticotropin-releasing factor/urotensin I family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 3p21.3. Cellular Component: extracellular space; extracellular region. Molecular Function: hormone binding; G-protein-coupled receptor binding; hormone activity; corticotropin-releasing hormone receptor 2 binding; receptor binding. Biological Process: negative regulation of luteinizing hormone secretion; cell proliferation; cellular response to nutrient levels; digestion; response to stress; cAMP biosynthetic process; G-protein signaling, adenylate cyclase activating pathway; negative regulation of follicle-stimulating hormone secretion

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800