ELISA/assay

Human chitinase 1 (chitotriosidase) , CHIT1 ELISA Kit

MBS923372

48-Strip-Wells

510 USD

ELISA Kit

Human chitinase 1 (chitotriosidase) , CHIT1 ELISA Kit

chitinase 1 (chitotriosidase)

Human chitinase 1 (chitotriosidase) (CHIT1) ELISA kit; CHI3; CHIT; FLJ00314; MGC125322; chitotriosidase; plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase; chitinase 1 (chitotriosidase)

chitotriosidase-1 isoform 2; Chitotriosidase-1; chitotriosidase-1; plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase; chitinase 1 (chitotriosidase); Chitinase-1

CHIT1

CHIT1; CHIT1; CHI3; CHIT; CHITD

CHIT1_HUMAN

Human

447

This assay has high sensitivity and excellent specificity for detection of human CHIT1. No significant cross-reactivity or interference between human CHIT1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, cell culture supernates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: 7.8 pg /ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CHIT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CHIT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHIT1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHIT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

388890228

NP_001243054.2

NM_001256125.1

Q13231

51,681 Da

Amino Sugar And Nucleotide Sugar Metabolism Pathway (82979); Amino Sugar And Nucleotide Sugar Metabolism Pathway (350)

Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jan 2012]

CHIT1: Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity. Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted; EC 3.2.1.14; Hydrolase; Carbohydrate Metabolism - amino sugar and nucleotide sugar. Chromosomal Location of Human Ortholog: 1q32.1. Cellular Component: extracellular space; lysosome. Molecular Function: endochitinase activity; chitin binding; chitinase activity. Biological Process: polysaccharide catabolic process; response to bacterium; immune response; chitin catabolic process. Disease: Chitotriosidase Deficiency

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800