ELISA/assay

Mouse Protein delta homolog 1, DLK1 ELISA Kit

MBS923350

48-Strip-Wells

510 USD

ELISA Kit

Mouse Protein delta homolog 1, DLK1 ELISA Kit

delta-like 1 homolog (Drosophila)

Mouse Protein delta homolog 1 (DLK1) ELISA kit; DLK; FA1; PREF1; Pref-1; ZOG; pG2; delta-like 1 homolog; delta-like homolog; fetal antigen 1; preadipocyte factor 1; secredeltin; delta-like 1 homolog (Drosophila)

protein delta homolog 1 isoform 1; Protein delta homolog 1; protein delta homolog 1; fetal antigen 1; preadipocyte factor 1; stromal cell derived protein 1; adipocyte differentiation inhibitor protein; delta-like 1 homolog (Drosophila); Adipocyte differentiation inhibitor protein; Preadipocyte factor 1; Pref-1

DLK1

Dlk1; Dlk1; FA1; ZOG; pG2; DlkI; Peg9; SCP1; DLK-1; Ly107; pref-1; AW742678; Dlk; Pref1; Scp-1; DLK-1; Pref-1; FA1

DLK1_MOUSE

Mouse

385

This assay has high sensitivity and excellent specificity for detection of mouse DLK1. No significant cross-reactivity or interference between mouse DLK1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of mouse DLK1 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DLK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DLK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLK1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

83816918

NP_034182.2

NM_010052.5

Q09163

41,320 Da

Adipogenesis Pathway (198299)

DLK1: May have a role in neuroendocrine differentiation. Monomer. Found within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Cellular Component: membrane; integral to membrane; external side of plasma membrane. Molecular Function: calcium ion binding. Biological Process: embryonic development; regulation of gene expression; embryonic skeletal development; negative regulation of Notch signaling pathway; cell differentiation; post-embryonic development

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800