ELISA/assay

Mouse Apoptosis-associated speck-like protein containing a CARD, PYCARD ELISA Kit

MBS923261

48-Strip-Wells

510 USD

ELISA Kit

Mouse Apoptosis-associated speck-like protein containing a CARD, PYCARD ELISA Kit

PYD and CARD domain containing

Mouse Apoptosis-associated speck-like protein containing a CARD (PYCARD) ELISA kit; ASC; CARD5; MGC10332; TMS; TMS-1; TMS1; apoptosis-associated speck-like protein containing a CARD; caspase recruitment domain protein 5; target of methylation-induced silencing-1; PYD and CARD domain containing

apoptosis-associated speck-like protein containing a CARD; Apoptosis-associated speck-like protein containing a CARD; apoptosis-associated speck-like protein containing a CARD; PYD and CARD domain-containing protein; PYD and CARD domain containing; PYD and CARD domain-containing protein

PYCARD

Pycard; Pycard; Asc; TNS1; masc; CARD5; TMS-1; 9130417A21Rik; Asc; mASC

ASC_MOUSE

Mouse

193

This assay has high sensitivity and excellent specificity for detection of mouse PYCARD. No significant cross-reactivity or interference between mouse PYCARD and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 7.81 pg/ml -500 pg/ml. Sensitivity: The minimum detectable dose of mouse PYCARD is typically less than 1.95 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PYCARD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PYCARD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PYCARD is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PYCARD bound in the initial step. The color development is stopped and the intensity of the color is measured.

165377185

NP_075747.3

NM_023258.4

Q9EPB4

21,459 Da

Cytosolic DNA-sensing Pathway (125146); Cytosolic DNA-sensing Pathway (124833); Influenza A Pathway (217174); Influenza A Pathway (217150); Legionellosis Pathway (469202); Legionellosis Pathway (469186); NOD-like Receptor Signaling Pathway (122195); NOD-like Receptor Signaling Pathway (122131); Pertussis Pathway (218112); Pertussis Pathway (218099)

PYCARD: Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. Forms complexes with other DAPIN domain-containing proteins. Interacts with CIAS1/PYPAF1 and PYDC1. Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U-937, but not in Jurkat T- cell lymphoma and Daudi Burkitt s lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and MOLT-4 lymphocytic leukemia cells. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Tumor suppressor; Adaptor/scaffold. Cellular Component: protein complex; mitochondrion; cell soma; endoplasmic reticulum; cytoplasm; extracellular region; nucleolus; IkappaB kinase complex; cytosol; nucleus. Molecular Function: protein binding; protein homodimerization activity; protease binding; apoptotic protease activator activity; Pyrin domain binding. Biological Process: myeloid dendritic cell activation during immune response; apoptosis; positive regulation of apoptosis; regulation of protein stability; positive regulation of caspase activity; positive regulation of JNK cascade; positive regulation of interleukin-1 beta secretion; defense response to Gram-negative bacterium; regulation of caspase activity; positive regulation of activated T cell proliferation; activation of NF-kappaB transcription factor; regulation of apoptosis; tumor necrosis factor-mediated signaling pathway; positive regulation of phagocytosis; inflammatory response; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; defense response to virus; positive regulation of adaptive immune response; caspase activation; regulation of GTPase activity; immune system process; positive regulation of interleukin-6 production; interleukin-1 beta production; negative regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of tumor necrosis factor production; negative regulation of interferon-beta production; myeloid dendritic cell activation; positive regulation of interferon-gamma production; positive regulation of actin filament polymerization; response to bacterium; inhibition of NF-kappaB transcription factor; regulation of inflammatory response; innate immune response; positive regulation of transcription factor activity; regulation of autophagy; positive regulation of T cell activation; positive regulation of defense response to virus by host; activation of innate immune response; positive regulation of antigen processing and presentation of peptide antigen via MHC class II

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800