ELISA/assay

Mouse Protein Wnt-2, WNT2 ELISA Kit

MBS923245

48-Strip-Wells

510 USD

ELISA Kit

Mouse Protein Wnt-2, WNT2 ELISA Kit

wingless-type MMTV integration site family member 2

Mouse Protein Wnt-2 (WNT2) ELISA kit; INT1L1; IRP; Int-1-related protein; protein Wnt-2; secreted growth factor; wingless-type MMTV integration site family member 2

protein Wnt-2; Protein Wnt-2; protein Wnt-2; INT-1-related protein; wingless-related MMTV integration site 2; wingless-type MMTV integration site family, member 2; INT-1-related protein; IRP

WNT2

Wnt2; Wnt2; Irp; Mirp; Wnt-2; Wnt2a; Int1l1; 2610510E18Rik; Irp; Wnt-2; IRP

WNT2_MOUSE

Mouse

360

This assay has high sensitivity and excellent specificity for detection of Mouse WNT2. No significant cross-reactivity or interference between Mouse WNT2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: 3.9 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Signaling Pathway. Protein Biological Process 3: Wnt signaling pathway

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for WNT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

242397432

NP_076142.3

NM_023653.5

P21552

40,482 Da

Basal Cell Carcinoma Pathway (83306); Basal Cell Carcinoma Pathway (525); Class B/2 (Secretin Family Receptors) Pathway (575448); ESC Pluripotency Pathways (198374); GPCR Ligand Binding Pathway (575425); HTLV-I Infection Pathway (373903); HTLV-I Infection Pathway (373889); Hedgehog Signaling Pathway (83260); Hedgehog Signaling Pathway (474); Melanogenesis Pathway (83289)

WNT2: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Belongs to the Wnt family. Protein type: Secreted; Cell development/differentiation; Extracellular matrix; Secreted, signal peptide; Cell adhesion. Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; cytoplasm; extracellular region; plasma membrane. Molecular Function: frizzled binding; cytokine activity; receptor binding. Biological Process: neuron differentiation; positive regulation of fibroblast proliferation; Wnt receptor signaling pathway; cell-cell signaling; atrial cardiac muscle morphogenesis; multicellular organismal development; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; positive regulation of transcription factor activity; Wnt receptor signaling pathway through beta-catenin; positive regulation of cardiac muscle cell proliferation

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800