product summary
Loading...
company name :
MyBioSource
product type :
ELISA/assay
product name :
Human Wnt inhibitory factor 1, WIF1 ELISA Kit
catalog :
MBS923202
quantity :
48-Strip-Wells
price :
510 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS923202
products type :
ELISA Kit
products full name :
Human Wnt inhibitory factor 1, WIF1 ELISA Kit
products short name :
WNT inhibitory factor 1
products name syn :
Human Wnt inhibitory factor 1 (WIF1) ELISA kit; WIF-1; ; WNT inhibitory factor 1
other names :
wnt inhibitory factor 1; Wnt inhibitory factor 1; wnt inhibitory factor 1; WNT inhibitory factor 1
products gene name :
WIF1
other gene names :
WIF1; WIF1; WIF-1; UNQ191/PRO217; WIF-1
uniprot entry name :
WIF1_HUMAN
reactivity :
Human
sequence length :
379
specificity :
This assay has high sensitivity and excellent specificity for detection of human WIF1. No significant cross-reactivity or interference between human WIF1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 47 pg/ml -3000 pg/ml. Sensitivity: The minimum detectable dose of human WIF1 is typically less than 11.7 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for WIF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WIF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WIF1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WIF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
111125011
ncbi acc num :
NP_009122.2
ncbi gb acc num :
NM_007191.4
uniprot acc num :
Q9Y5W5
ncbi mol weight :
41,528 Da
ncbi pathways :
Presenilin Action In Notch And Wnt Signaling Pathway (137914); Wnt Signaling Network Pathway (137962); Wnt Signaling Pathway (83061); Wnt Signaling Pathway (471)
ncbi summary :
The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. This protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers. [provided by RefSeq, Jun 2010]
uniprot summary :
WIF1: Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation. Protein type: Secreted, signal peptide; Secreted; Inhibitor. Chromosomal Location of Human Ortholog: 12q14.3. Cellular Component: extracellular region. Molecular Function: protein binding. Biological Process: Wnt receptor signaling pathway; multicellular organismal development; positive regulation of fat cell differentiation; signal transduction
size1 :
48-Strip-Wells
price1 :
510 USD
size2 :
96-Strip-Wells
price2 :
725
size3 :
5x96-Strip-Wells
price3 :
2565
size4 :
10x96-Strip-Wells
price4 :
4800
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

Would you like to receive email and e-newsletter from MyBioSource about new products, special offers and events? Please click here to join our Mailing List!