ELISA/assay

Human Methylenetetrahydrofolate reductase, MTHFR ELISA Kit

MBS923196

48-Strip-Wells

510 USD

ELISA Kit

Human Methylenetetrahydrofolate reductase, MTHFR ELISA Kit

5, 10-methylenetetrahydrofolate reductase (NADPH)

Human Methylenetetrahydrofolate reductase (MTHFR) ELISA kit; 5; 10-methylenetetrahydrofolate reductase; OTTHUMP00000002367; 5; 10-methylenetetrahydrofolate reductase (NADPH)

methylenetetrahydrofolate reductase; Methylenetetrahydrofolate reductase; methylenetetrahydrofolate reductase; 5,10-methylenetetrahydrofolate reductase (NADPH); methylenetetrahydrofolate reductase (NAD(P)H)

MTHFR

MTHFR; MTHFR

MTHR_HUMAN

Human

656

This assay has high sensitivity and excellent specificity for detection of human MTHFR. No significant cross-reactivity or interference between human MTHFR and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 46.88 pg/ml -3000 pg/ml. Sensitivity: The minimum detectable dose of human MTHFR is typically less than 11.7 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MTHFR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MTHFR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTHFR is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTHFR bound in the initial step. The color development is stopped and the intensity of the color is measured.

87240000

NP_005948.3

NM_005957.4

P42898

74,597 Da

Fluoropyrimidine Activity Pathway (198778); Folate Metabolism Pathway (198833); Metabolism Pathway (477135); Metabolism Of Folate And Pterines Pathway (106260); Metabolism Of Vitamins And Cofactors Pathway (106249); Metabolism Of Water-soluble Vitamins And Cofactors Pathway (106250); One Carbon Metabolism Pathway (198756); One Carbon Pool By Folate Pathway (83008); One Carbon Pool By Folate Pathway (393); Selenium Pathway (198825)

The protein encoded by this gene catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine. Genetic variation in this gene influences susceptibility to occlusive vascular disease, neural tube defects, colon cancer and acute leukemia, and mutations in this gene are associated with methylenetetrahydrofolate reductase deficiency.[provided by RefSeq, Oct 2009]

MTHFR: Catalyzes the conversion of 5,10- methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co- substrate for homocysteine remethylation to methionine. Defects in MTHFR are the cause of methylenetetrahydrofolate reductase deficiency (MTHFRD). MTHFRD is autosomal recessive disorder with a wide range of features including homocysteinuria, homocysteinemia, developmental delay, severe mental retardation, perinatal death, psychiatric disturbances, and later-onset neurodegenerative disorders. Defects in MTHFR may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Defects in MTHFR may be a cause of susceptibility to folate-sensitive neural tube defects (FS-NTD). The most common NTDs are open spina bifida (myelomeningocele) and anencephaly. Belongs to the methylenetetrahydrofolate reductase family. Protein type: Cofactor and Vitamin Metabolism - one carbon pool by folate; Energy Metabolism - methane; Oxidoreductase; EC 1.5.1.20. Chromosomal Location of Human Ortholog: 1p36.3. Cellular Component: neuron projection; cytosol. Molecular Function: FAD binding; NADP binding; methylenetetrahydrofolate reductase (NADPH) activity. Biological Process: response to drug; amino acid metabolic process; vitamin metabolic process; methionine biosynthetic process; blood circulation; response to folic acid; response to vitamin B2; response to hypoxia; S-adenosylmethionine metabolic process; homocysteine metabolic process; one-carbon compound metabolic process; folic acid metabolic process; water-soluble vitamin metabolic process. Disease: Schizophrenia; Thrombophilia Due To Thrombin Defect; Neural Tube Defects, Folate-sensitive; Homocystinuria Due To Deficiency Of N(5,10)-methylenetetrahydrofolate Reductase Activity

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800