catalog number :
MBS922852
products type :
ELISA Kit
products full name :
Human Ribonuclease 7, RNASE7 ELISA Kit
products short name :
ribonuclease, RNase A family, 7
products name syn :
Human Ribonuclease 7 (RNASE7) ELISA kit; MGC133220; ribonuclease 7; ribonuclease; RNase A family; 7
other names :
ribonuclease 7; Ribonuclease 7; ribonuclease 7; SAP-2; RNase 7; skin-derived antimicrobial protein 2; ribonuclease, RNase A family, 7; Skin-derived antimicrobial protein 2; SAP-2
products gene name :
RNASE7
other gene names :
RNASE7; RNASE7; UNQ2516/PRO6006; RNase 7; SAP-2
uniprot entry name :
RNAS7_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human RNASE7. No significant cross-reactivity or interference between human RNASE7 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, urine, saliva, urine, saliva, urine, saliva, urine, saliva, urine, saliva, urine, saliva, urine, saliva, urine, saliva, urine, saliva, urine, saliva, urine, saliva, urine, saliva, cell culture supernates, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 1.56 ng/ml -100 ng/ml. Sensitivity: 0.39 ng /ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RNASE7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RNASE7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RNASE7 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RNASE7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_115961.2
ncbi gb acc num :
NM_032572.3
ncbi mol weight :
17,419 Da
ncbi summary :
RNASE7 is a member of the RNase A superfamily that has broad-spectrum antimicrobial activity and ribonuclease activity (Harder and Schroder, 2002 [PubMed 12244054]; Zhang et al., 2003 [PubMed 12527768]).[supplied by OMIM, Dec 2008]
uniprot summary :
RNASE7: Exhibits a potent RNase activity. Has broad-spectrum antimicrobial activity against many pathogenic microorganisms and remarkably potent activity (lethal dose of 90% 30 nM) against a vancomycin resistant Enterococcus faecium. Belongs to the pancreatic ribonuclease family. Protein type: Secreted; RNA-binding; EC 3.1.27.-; Ribonuclease; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 14q11.2. Cellular Component: extracellular region. Molecular Function: peptidoglycan binding; nucleic acid binding; lipopolysaccharide binding; endonuclease activity; ribonuclease activity. Biological Process: defense response to Gram-positive bacterium; response to bacterium; antibacterial humoral response; innate immune response; membrane disruption in another organism; defense response to Gram-negative bacterium
size4 :
10x96-Strip-Wells