ELISA/assay

Mouse EGF-like repeat and discoidin I-like domain-containing protein 3, EDIL3 ELISA Kit

MBS922828

48-Strip-Wells

510 USD

ELISA Kit

Mouse EGF-like repeat and discoidin I-like domain-containing protein 3, EDIL3 ELISA Kit

EGF-like repeats and discoidin I-like domains 3

Mouse EGF-like repeat and discoidin I-like domain-containing protein 3 (EDIL3) ELISA kit; DEL1; MGC26287; EGF-like repeats and discoidin I-like domains-containing protein 3; developmental endothelial locus-1; EGF-like repeats and discoidin I-like domains 3

EGF-like repeat and discoidin I-like domain-containing protein 3 isoform a; EGF-like repeat and discoidin I-like domain-containing protein 3; EGF-like repeat and discoidin I-like domain-containing protein 3; integrin-binding protein DEL1; developmental endothelial locus-1; developmentally-regulated endothelial cell locus 1 protein; EGF-like repeats and discoidin I-like domains 3; Developmentally-regulated endothelial cell locus 1 protein; Integrin-binding protein DEL1

EDIL3

Edil3; Edil3; Del1; Del-1; Del1

EDIL3_MOUSE

Mouse

480

This assay has high sensitivity and excellent specificity for detection of mouse EDIL3. No significant cross-reactivity or interference between mouse EDIL3 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates and tissue homogenates. Assay Type: Sandwich. Detection Range: 28 pg/ml -1800 pg/ml. Sensitivity: The minimum detectable dose of mouse EDIL3 is typically less than 7 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EDIL3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EDIL3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EDIL3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EDIL3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

83921564

NP_001033076.1

NM_001037987.3

O35474

53,712 Da

EDIL3: Promotes adhesion of endothelial cells through interaction with the alpha-v/beta-3 integrin receptor. Inhibits formation of vascular-like structures. May be involved in regulation of vascular morphogenesis of remodeling in embryonic development. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Cell adhesion; Secreted. Cellular Component: extracellular region. Molecular Function: integrin binding; metal ion binding; calcium ion binding. Biological Process: multicellular organismal development; cell adhesion

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800