protein

RAB7 Antibody (C-term) Blocking Peptide

MBS9227775

0.1 mg

155 USD

Blocking Peptide

RAB7 Antibody (C-term) Blocking Peptide

[RAB7]

[RAB7]

[Ras-related protein Rab-7a; Ras-related protein Rab-7a; ras-related protein Rab-7a; RAB7A, member RAS oncogene family]

[RAB7A]

[RAB7]

[RAB7A; RAB7A; RAB7; PRO2706; RAB7]

RAB7A_HUMAN

[90504025]

207

The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human RAB7. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Cellular Location: Cytoplasmic vesicle, phagosome membrane;Peripheral membrane protein; Cytoplasmicside. Late endosome membrane; Peripheralmembrane protein; Cytoplasmic side.Lysosome membrane; Peripheral membraneprotein; Cytoplasmic side. Melanosomemembrane; Peripheral membrane protein;Cytoplasmic side. Cytoplasmic vesicle,autophagosome membrane; Peripheralmembrane protein; Cytoplasmic side. Lipiddroplet {ECO:0000250 UniProtKB:P51150}.Endosome membrane. Note=Colocalizes withOSBPL1A at the late endosome(PubMed:16176980). Found in the ruffledborder (a late endosomal-like compartment inthe plasma membrane) of bone-resorbingosteoclasts. Recruited to phagosomescontaining S.aureus or Mycobacterium(PubMed:21255211). Lipid droplet localizationis increased upon ADRB2 stimulation (By similarity) {ECO:0000250 UniProtKB:P51150, ECO:0000269 PubMed:16176980, ECO:0000269 PubMed:21255211}. Tissue Location: Widely expressed; high expression found in skeletal muscle.

Background: RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. RAB7 is a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Function: Key regulator in endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not RAB7 Antibody (C-term) Blocking Peptide - Background RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. RAB7 is a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. RAB7 Antibody (C-term) Blocking Peptide - References Davies,J.P., et.al., Genomics 41 (1), 131-134 (1997)Vitelli,R., et.al., Biochem. Biophys. Res. Commun. 229 (3), 887-890 (1996) only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient- transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA (PubMed: 11179213 , PubMed: 12944476 ,PubMed: 14617358 , PubMed: 20028791 ,PubMed: 21255211 ). Regulatesthe endocytic trafficking of the EGF- EGFRcomplex by regulating its lysosomaldegradation. Involved in the ADRB2-stimulated lipolysis through lipophagy, a cytosolic lipase-independent autophagic pathway (By similarity). Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed: 22660413 ).

1709999

P51149.1

P51149

Adaptive Immune System Pathway (1269171); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Endocytosis Pathway (102279); Endocytosis Pathway (102181); IL12-mediated Signaling Events Pathway (137922); Immune System Pathway (1269170); MHC Class II Antigen Presentation Pathway (1269200); Phagosome Pathway (153910); Phagosome Pathway (153859)

RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies. [provided by RefSeq, Jul 2008]

Rab7: a member of the RAB family of RAS-related GTP-binding proteins, important regulators of vesicular transport and are located in specific intracellular compartments. RAB7 has been localized to late endosomes and shown to be important in the late endocytic pathway. In addition, it has been shown to have a fundamental role in the cellular vacuolation induced by the cytotoxin VacA of Helicobacter pylori. Mutations in this protein cause Charcot-Marie-Tooth type 2B neuropathy. Protein type: G protein, monomeric; G protein, monomeric, Rab; G protein. Chromosomal Location of Human Ortholog: 3q21.3. Cellular Component: intracellular membrane-bound organelle; late endosome; late endosome membrane; lipid particle; lysosomal membrane; lysosome; phagocytic vesicle; retromer complex; vacuolar membrane. Molecular Function: GDP binding; GTP binding; GTPase activity; protein binding. Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; early endosome to late endosome transport; endocytosis; endosome to lysosome transport; epidermal growth factor ligand processing; positive regulation of protein catabolic process; positive regulation of viral reproduction; protein targeting to lysosome; protein to membrane docking; protein transport; release of virus from host; retrograde transport, endosome to Golgi. Disease: Charcot-marie-tooth Disease, Axonal, Type 2b

0.1 mg

155 USD