catalog number :
MBS922746
products type :
ELISA Kit
products full name :
Monkey Brain-derived neurotrophic factor, BDNF ELISA Kit
products short name :
brain-derived neurotrophic factor
products name syn :
Monkey Brain-derived neurotrophic factor (BDNF) ELISA kit; MGC34632; neurotrophin; brain-derived neurotrophic factor
other names :
Brain-derived neurotrophic factor; Brain-derived neurotrophic factor; brain-derived neurotrophic factor
products gene name :
BDNF
other gene names :
BDNF; BDNF; BDNF
uniprot entry name :
BDNF_MACMU
specificity :
This assay has high sensitivity and excellent specificity for detection of Monkey BDNF. No significant cross-reactivity or interference between Monkey BDNF and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.78 ng/ml-50 ng/ml. Sensitivity: 0.195 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BDNF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BDNF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BDNF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BDNF bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
12,956 Da
ncbi pathways :
Alcoholism Pathway (585573); Alcoholism Pathway (587116); Cocaine Addiction Pathway (546265); Cocaine Addiction Pathway (546273); Huntington's Disease Pathway (86770); Huntington's Disease Pathway (512); MAPK Signaling Pathway (86718); MAPK Signaling Pathway (456); Neurotrophin Signaling Pathway (101163); Neurotrophin Signaling Pathway (100064)
uniprot summary :
Function: During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS
size4 :
10x96-Strip-Wells