Rat Membrane primary amine oxidase, AOC3 ELISA Kit | MyBioSource MBS922741 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Membrane primary amine oxidase, AOC3 ELISA Kit

catalog :

MBS922741

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS922741

products type :

ELISA Kit

products full name :

Rat Membrane primary amine oxidase, AOC3 ELISA Kit

products short name :

amine oxidase, copper containing 3 (vascular adhesion protein 1)

products name syn :

Rat Membrane primary amine oxidase (AOC3) ELISA kit; HPAO; SSAO; VAP-1; VAP1; amine oxidase; copper containing 3; copper amine oxidase; semicarbazide-sensitive amine oxidase; vascular adhesion protein 1; amine oxidase; copper containing 3 (vascular adhesion protein 1)

other names :

membrane primary amine oxidase; Membrane primary amine oxidase; membrane primary amine oxidase; VP97; VAP-1; copper amine oxidase; vascular adhesion protein 1; semicarbazide-sensitive amine oxidase; amine oxidase, copper containing 3; Copper amine oxidase; Semicarbazide-sensitive amine oxidase; SSAO; VP97; Vascular adhesion protein 1; VAP-1

products gene name :

AOC3

other gene names :

Aoc3; Aoc3; SSAO; SSAO; VAP-1

uniprot entry name :

AOC3_RAT

reactivity :

Rat

sequence length :

763

specificity :

This assay has high sensitivity and excellent specificity for detection of rat AOC3. No significant cross-reactivity or interference between rat AOC3 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 7.8 ng/ml -500 ng/ml. Sensitivity: The minimum detectable dose of rat AOC3 is typically less than 1.95 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AOC3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AOC3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AOC3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AOC3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

71896590

ncbi acc num :

NP_113770.2

ncbi gb acc num :

NM_031582.2

uniprot acc num :

O08590

ncbi mol weight :

84,981 Da

ncbi pathways :

Glycine, Serine And Threonine Metabolism Pathway (83342); Glycine, Serine And Threonine Metabolism Pathway (313); Glycolysis And Gluconeogenesis Pathway (198445); Phenylalanine Metabolism Pathway (83352); Phenylalanine Metabolism Pathway (327); Proteasome Degradation Pathway (198435); Tyrosine Metabolism Pathway (83351); Tyrosine Metabolism Pathway (325); Beta-Alanine Metabolism Pathway (83358); Beta-Alanine Metabolism Pathway (335)

ncbi summary :

localized to the adipocyte plasma membrane and to glucose transporter Glut4 containing vesicles [RGD, Feb 2006]

uniprot summary :

AOC3: Cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin- independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis. Belongs to the copper/topaquinone oxidase family. Protein type: Cell surface; Calcium-binding; Membrane protein, integral; Oxidoreductase; Cell adhesion; Motility/polarity/chemotaxis; Other Amino Acids Metabolism - beta-alanine; Amino Acid Metabolism - phenylalanine; EC 1.4.3.21; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - glycine, serine and threonine. Cellular Component: extracellular space; cell surface; microvillus; intracellular membrane-bound organelle; cytoplasm; plasma membrane; integral to membrane. Molecular Function: amine oxidase activity; protein homodimerization activity; copper ion binding; protein heterodimerization activity; cation channel activity; calcium ion binding; quinone binding. Biological Process: response to antibiotic; eating behavior; positive regulation of acute inflammatory response; positive regulation of leukocyte migration; regulation of blood pressure; amine metabolic process; cell adhesion; cation transport; leukocyte migration during inflammatory response; aging

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage