Human Nuclear factor erythroid 2-related factor 2, NFE2L2 ELISA Kit | MyBioSource MBS922594 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Nuclear factor erythroid 2-related factor 2, NFE2L2 ELISA Kit

catalog :

MBS922594

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS922594

products type :

ELISA Kit

products full name :

Human Nuclear factor erythroid 2-related factor 2, NFE2L2 ELISA Kit

products short name :

nuclear factor (erythroid-derived 2) -like 2

products name syn :

Human Nuclear factor erythroid 2-related factor 2 (NFE2L2) ELISA kit; NRF2; NF-E2-related factor 2; nuclear factor erythroid 2-like 2; nuclear factor (erythroid-derived 2) -like 2

other names :

nuclear factor erythroid 2-related factor 2 isoform 2; Nuclear factor erythroid 2-related factor 2; nuclear factor erythroid 2-related factor 2; HEBP1; NFE2-related factor 2; NF-E2-related factor 2; nuclear factor erythroid-derived 2-like 2; nuclear factor, erythroid derived 2, like 2; nuclear factor, erythroid 2-like 2; HEBP1; Nuclear factor, erythroid derived 2, like 2

products gene name :

NFE2L2

other gene names :

NFE2L2; NFE2L2; NRF2; NRF2; NF-E2-related factor 2; NFE2-related factor 2

uniprot entry name :

NF2L2_HUMAN

reactivity :

Human

sequence length :

589

specificity :

This assay has high sensitivity and excellent specificity for detection of Human NFE2L2. No significant cross-reactivity or interference between Human NFE2L2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, urine, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml-2000 pg/ml. Sensitivity: 7.8 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Transcription/Transcription regulation. Protein Biological Process 3: Transcription

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NFE2L2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NFE2L2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NFE2L2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NFE2L2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

224028257

ncbi acc num :

NP_001138884.1

ncbi gb acc num :

NM_001145412.2

uniprot acc num :

Q16236

ncbi mol weight :

67,827 Da

ncbi pathways :

Keap1-Nrf2 Pathway (198777); Protein Processing In Endoplasmic Reticulum Pathway (167325); Protein Processing In Endoplasmic Reticulum Pathway (167190)

ncbi summary :

This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

uniprot summary :

NRF2: a transcription factor that regulates basal expression and antioxidant induction of a transcription factor for NAD(P)H:quinone oxidoreductase-1 (NQO1) and other detoxifying genes. Targeted for proteasomal degradation by INrf2. Oxidative stress causes its phosphorylation and release from INrf2. Protein type: DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 2q31. Cellular Component: nucleoplasm; centrosome; cytoplasm; plasma membrane; cytosol; nucleus; chromatin. Molecular Function: protein domain specific binding; protein binding; DNA binding; transcription factor activity. Biological Process: transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; positive regulation of blood coagulation; unfolded protein response; regulation of embryonic development; proteasomal ubiquitin-independent protein catabolic process; protein ubiquitination; positive regulation of transcription from RNA polymerase II promoter

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage