catalog number :
MBS922413
products type :
ELISA Kit
products full name :
Pig Beta-1 adrenergic receptor, ADRB1 ELISA Kit
products short name :
adrenergic, beta-1-, receptor
products name syn :
Pig Beta-1 adrenergic receptor (ADRB1) ELISA kit; ADRB1R; B1AR; BETA1AR; RHR; beta-1-adrenergic receptor; adrenergic; beta-1-; receptor
other names :
beta-1 adrenergic receptor; Beta-1 adrenergic receptor; beta-1 adrenergic receptor; beta-1 adrenoceptor; beta-1 adrenoreceptor; beta 1 adrenergic protein; adrenergic, beta-1-, receptor; Beta-1 adrenoreceptor; Beta-1 adrenoceptor
products gene name :
ADRB1
other gene names :
ADRB1; ADRB1; Beta-1 adrenoceptor
uniprot entry name :
ADRB1_PIG
specificity :
This assay has high sensitivity and excellent specificity for detection of Pig ADRB1. No significant cross-reactivity or interference between Pig ADRB1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml-2000 pg/ml. Sensitivity: 7.81 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADRB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADRB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADRB1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADRB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001116546.1
ncbi gb acc num :
NM_001123074.1
ncbi mol weight :
50,098 Da
ncbi pathways :
Calcium Signaling Pathway (84462); Calcium Signaling Pathway (459); Dilated Cardiomyopathy Pathway (121477); Dilated Cardiomyopathy Pathway (121285); Endocytosis Pathway (102268); Endocytosis Pathway (102181); Gap Junction Pathway (84484); Gap Junction Pathway (483); Neuroactive Ligand-receptor Interaction Pathway (84465); Neuroactive Ligand-receptor Interaction Pathway (462)
uniprot summary :
Function: Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity. Mediates Ras activation through G(s)-alpha- and cAMP-mediated signaling
size4 :
10x96-Strip-Wells