catalog number :
MBS922364
products type :
ELISA Kit
products full name :
Human Tetranectin, CLEC3B ELISA Kit
products short name :
C-type lectin domain family 3, member B
products name syn :
Human Tetranectin (CLEC3B) ELISA kit; DKFZp686H17246; TN; TNA; tetranectin (plasminogen binding protein) ; tetranectin (plasminogen-binding protein) ; C-type lectin domain family 3; member B
other names :
tetranectin; Tetranectin; tetranectin; plasminogen kringle 4-binding protein; tetranectin (plasminogen binding protein); tetranectin (plasminogen-binding protein); C-type lectin domain family 3, member B; C-type lectin domain family 3 member B; Plasminogen kringle 4-binding protein
products gene name :
CLEC3B
other gene names :
CLEC3B; CLEC3B; TN; TNA; TNA; TN
uniprot entry name :
TETN_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human CLEC3B. No significant cross-reactivity or interference between human CLEC3B and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma and tissue homogenates. Assay Type: Sandwich. Detection Range: 4. 7 ng/ml -300 ng/ml. Sensitivity: The minimum detectable dose of human CLEC3B is typically less than 1.17 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CLEC3B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CLEC3B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CLEC3B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CLEC3B bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_003269.2
ncbi gb acc num :
NM_003278.2
ncbi mol weight :
22,537 Da
uniprot summary :
CLEC3B: Tetranectin binds to plasminogen and to isolated kringle 4. May be involved in the packaging of molecules destined for exocytosis. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 3p22-p21.3. Cellular Component: extracellular matrix; extracellular space; granular component; cytoplasm; extracellular region. Molecular Function: heparin binding; calcium ion binding; carbohydrate binding. Biological Process: ossification; skeletal development; bone mineralization
size4 :
10x96-Strip-Wells