ELISA/assay

Rat Cathepsin L1, CTSL1 ELISA Kit

MBS922239

48-Strip-Wells

510 USD

ELISA Kit

Rat Cathepsin L1, CTSL1 ELISA Kit

cathepsin L1

Rat Cathepsin L1 (CTSL1) ELISA kit; CATL; CTSL; FLJ31037; MEP; cathepsin L; major excreted protein; cathepsin L1

cathepsin L1 preproprotein; Cathepsin L1; cathepsin L1; MEP; CP-2; cyclic protein 2; major excreted protein; cathepsin L; Cathepsin L; Cyclic protein 2; CP-2; Major excreted protein; MEP

CTSL1

Ctsl; Ctsl; CatL; CATHL; Ctsl1; Ctsl1; CP-2; MEP

CATL1_RAT

Rat

334

This assay has high sensitivity and excellent specificity for detection of Rat CTSL1. No significant cross-reactivity or interference between Rat CTSL1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 6.25 pg/ml-400 pg/ml. Sensitivity: 1.56 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CTSL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CTSL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CTSL1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CTSL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

6978723

NP_037288.1

NM_013156.2

P07154

37,660 Da

Antigen Processing And Presentation Pathway (83466); Antigen Processing And Presentation Pathway (485); Endochondral Ossification Pathway (219771); Lysosome Pathway (98756); Lysosome Pathway (96865); Phagosome Pathway (153901); Phagosome Pathway (153859); Rheumatoid Arthritis Pathway (200306); Rheumatoid Arthritis Pathway (200269)

mouse homolog is deleted in the CD4 T-cell-deficient mouse mutant nacht (nkt); may play a role in CD4+ T-cell selection in the thymus [RGD, Feb 2006]

CTSL2: Cysteine protease. May have an important role in corneal physiology. Belongs to the peptidase C1 family. Protein type: Protease; Secreted; EC 3.4.22.15; Secreted, signal peptide. Cellular Component: extracellular space; neuron projection; microvillus; apical part of cell; lysosome; cytoplasm; nucleolus; perikaryon; vacuole; cytoplasmic vesicle; nucleus; secretory granule; external side of plasma membrane. Molecular Function: collagen binding; proteoglycan binding; histone binding; fibronectin binding; cysteine-type endopeptidase activity; protein complex binding; cysteine-type carboxypeptidase activity; aminopeptidase activity; kininogen binding; peptide binding; cysteine-type peptidase activity. Biological Process: adaptive immune response; hair follicle morphogenesis; male gonad development; response to glucocorticoid stimulus; Sertoli cell differentiation; multicellular organismal aging; decidualization; cellular response to starvation; proteolysis; response to organic cyclic substance; collagen catabolic process; protein autoprocessing; proteolysis involved in cellular protein catabolic process; response to glucose stimulus; autophagic cell death; cell communication; nerve development; spermatogenesis; protein processing

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800