ELISA/assay

Human RE1-silencing transcription factor, REST ELISA Kit

MBS922211

48-Strip-Wells

510 USD

ELISA Kit

Human RE1-silencing transcription factor, REST ELISA Kit

RE1-silencing transcription factor

Human RE1-silencing transcription factor (REST) ELISA kit; NRSF; XBR; neuron restrictive silencer factor; repressor binding to the X2 box; RE1-silencing transcription factor

RE1-silencing transcription factor; RE1-silencing transcription factor; RE1-silencing transcription factor; repressor binding to the X2 box; neural-restrictive silencer factor; neuron restrictive silencer factor; RE1-silencing transcription factor variant E1a/E2/E4; RE1-silencing transcription factor variant E1a/E2/E3/E5; RE1-silencing transcription factor variant E1b/E2/E3/E5; RE1-silencing transcription factor variant E1c/E2/E3/E5; RE1-silencing transcription factor variant E1a/E2/E3/E4c; RE1-silencing transcription factor variant E1c/E2g/E3/E4; RE1-silencing transcription factor variant E1a/E2/E3/N3c/E4; RE1-silencing transcription factor variant E1b/E2/E3/N3c/E4; RE1-silencing transcription factor variant E1a/E2/E3/N3a/E4i; RE1-silencing transcription factor variant E1b/E2/E3/N3b/E4i; RE1-silencing transcription factor variant E1b/E2c/E2j/E3/E4; RE1-silencing transcription factor variant E1a/E2k/E2i/E3/E4j; RE1-silencing transcription factor; Neural-restrictive silencer factor; X2 box repressor

REST

REST; REST; XBR; NRSF; NRSF; XBR

REST_HUMAN

Human

1097

This assay has high sensitivity and excellent specificity for detection of human REST. No significant cross-reactivity or interference between human REST and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: The minimum detectable dose of human REST is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for REST has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any REST present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for REST is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of REST bound in the initial step. The color development is stopped and the intensity of the color is measured.

301897977

NP_001180437.1

NM_001193508.1

Q13127

121,872 Da

Huntington's Disease Pathway (83100); Huntington's Disease Pathway (512); SIDS Susceptibility Pathways (198901)

This gene encodes a transcriptional repressor that represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regular of neurogenesis. Alternatively spliced transcript variants have been described [provided by RefSeq, Jul 2010]

REST: a transcriptional repressor that binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells during embryonic development. Induced during ageing in human cortical and hippocampal neurons, where it represses genes that promote cell death and induces the expression of stress response genes. Protects neurons from amyloid beta- and oxidative stress-induced toxicity. The deletion of REST in the mouse brain induces age-related neurodegeneration. Restricts the expression of neuronal genes by associating with two distinct corepressors, SIN3A and RCOR1, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. REST activates DYRK1A transcription via a neuron-restricted silencer element in the DYRK1A promoter. Four isoforms of the human protein are produced by alternative splicing. Protein type: Cell development/differentiation; Transcription factor; DNA-binding; C2H2-type zinc finger protein. Chromosomal Location of Human Ortholog: 4q12. Cellular Component: nucleoplasm; transcriptional repressor complex; cytosol; nucleus. Molecular Function: protein binding; outward rectifier potassium channel activity; metal ion binding; chromatin binding; transcription factor binding; transcription factor activity. Biological Process: transcription from RNA polymerase II promoter; negative regulation of cell proliferation; establishment and/or maintenance of chromatin architecture; negative regulation of neuron differentiation; negative regulation of calcium ion-dependent exocytosis; regulation of transcription, DNA-dependent; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; negative regulation of aldosterone biosynthetic process; positive regulation of caspase activity; hemopoietic progenitor cell differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of neurogenesis; negative regulation of insulin secretion

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800