catalog number :
MBS922180
products type :
ELISA Kit
products full name :
Mouse ADM2, ADM2 ELISA Kit
products short name :
adrenomedullin 2
products name syn :
Mouse ADM2 (ADM2) ELISA kit; RP4-579N16.10; AM2; FLJ21135; dJ579N16.4; OTTHUMP00000196596; intermedin; adrenomedullin 2
other names :
ADM2 preproprotein; ADM2; ADM2; intermedin; adrenomedullin 2; IntermedinCleaved into the following 2 chains:Adrenomedullin-2; AM2Alternative name(s):Intermedin-long; IMDL
products gene name :
ADM2
other gene names :
Adm2; Adm2; Am2; IMD; Imdn; Am2; AM2; IMDL; IMDS
uniprot entry name :
ADM2_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse ADM2. No significant cross-reactivity or interference between Mouse ADM2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm
other info2 :
Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADM2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADM2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_891558.1
ncbi gb acc num :
NM_182928.4
ncbi mol weight :
16,188 Da
ncbi pathways :
Calcitonin-like Ligand Receptors Pathway 575449!!Class B/2 (Secretin Family Receptors) Pathway 575448!!G Alpha (s) Signalling Events Pathway 575453!!GPCR Downstream Signaling Pathway 575452!!GPCR Ligand Binding Pathway 575425!!Signal Transduction Pathway 575318!!Signaling By GPCR Pathway 575424
uniprot summary :
Function: IMDL and IMDS may play a role as physiological regulators of gastrointestinal, cardiovascular bioactivities mediated by the CALCRL/RAMPs receptor complexes. Activates the cAMP-dependent pathway.2 PublicationsManual assertion based on experiment in:Ref.1