catalog number :
MBS921911
products type :
ELISA Kit
products full name :
Bovine CD63 antigen, CD63 ELISA Kit
products short name :
CD63 molecule
products name syn :
Bovine CD63 antigen (CD63) ELISA kit; LAMP-3; ME491; MLA1; OMA81H; TSPAN30; CD63 antigen; CD63 antigen (melanoma 1 antigen) ; granulophysin; lysosome-associated membrane glycoprotein 3; melanoma 1 antigen; melanoma-associated antigen ME491; me; CD63 molecule
other names :
CD63 antigen; CD63 antigen; CD63 antigen; CD63 molecule; CD_antigen: CD63
products gene name :
CD63
other gene names :
CD63; CD63
uniprot entry name :
CD63_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of Bovine CD63. No significant cross-reactivity or interference between Bovine CD63 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates and cell culture supernates. Assay Type: Sandwich. Detection Range: 1.87 ng/ml-120 ng/ml. Sensitivity: 0.47 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD63 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD63 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD63 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD63 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_991372.1
ncbi gb acc num :
NM_205803.1
ncbi mol weight :
25,752 Da
ncbi pathways :
Hemostasis Pathway (581607); Lysosome Pathway (98762); Lysosome Pathway (96865); Platelet Activation, Signaling And Aggregation Pathway (581617); Platelet Degranulation Pathway (581629); Response To Elevated Platelet Cytosolic Ca2+ Pathway (581627)
uniprot summary :
Function: Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli
size4 :
10x96-Strip-Wells