ELISA/assay

Human Glutathione synthetase, GSS ELISA Kit

MBS921757

96 Strip Wells

760 USD

ELISA Kit

Human Glutathione synthetase, GSS ELISA Kit

glutathione synthetase

Human Glutathione synthetase (GSS) ELISA kit; GSHS; MGC14098; GSH synthetase; glutathione synthase; glutathione synthetase

glutathione synthetase; Glutathione synthetase; glutathione synthetase; GSH-S; GSH synthetase; glutathione synthase; epididymis secretory sperm binding protein Li 64p; epididymis secretory sperm binding protein Li 88n; glutathione synthetase; Glutathione synthase

GSS

GSS; GSS; GSHS; HEL-S-64p; HEL-S-88n; GSH synthetase; GSH-S

GSHB_HUMAN

Human

474

This assay has high sensitivity and excellent specificity for detection of human GSS. No significant cross-reactivity or interference between human GSS and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of human glutathione synthetase (GSS) concentrations in serum, plasma, tissue homogenates. Detection Range: 0.156 U/ml -10 U/ml.

Sensitivity: The minimum detectable dose of human GSS is typically less than 0.039 U/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GSS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GSS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GSS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GSS bound in the initial step. The color development is stopped and the intensity of the color is measured.

4504169

NP_000169.1

NP_000169.1

P48637

52,385 Da

Biological Oxidations Pathway 105698!!Glutathione Biosynthesis, Glutamate = Glutathione Pathway 413393!!Glutathione Biosynthesis, Glutamate = Glutathione Pathway 468307!!Glutathione Conjugation Pathway 105727!!Glutathione Metabolism Pathway 82973!!Glutathione Metabolism Pathway 198824!!Glutathione Metabolism Pathway 343!!Glutathione Synthesis And Recycling Pathway 105728!!Metabolism Pathway 477135!!Phase II Conjugation Pathway 105721

Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency. [provided by RefSeq, Jul 2008]

96 Strip Wells

760 USD