MLL3 Antibody (C-term) | MyBioSource MBS9217235 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

MLL3 Antibody (C-term)

catalog :

MBS9217235

quantity :

0.05 mL

price :

150 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

ELISA, immunohistochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Staining MLL3 in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

product information

catalog number :

MBS9217235

products type :

Antibody

products full name :

MLL3 Antibody (C-term)

products short name :

[MLL3]

products name syn :

[Histone-lysine N-methyltransferase 2C; Lysine N-methyltransferase 2C; 2.1.1.43; Homologous to ALR protein; Myeloid/lymphoid or mixed-lineage leukemia protein 3; KMT2C; HALR; KIAA1506; MLL3]

other names :

[histone-lysine N-methyltransferase 2C; Histone-lysine N-methyltransferase 2C; histone-lysine N-methyltransferase 2C; lysine methyltransferase 2C; Homologous to ALR protein; Myeloid/lymphoid or mixed-lineage leukemia protein 3]

products gene name :

[KMT2C]

products gene name syn :

[HALR; KIAA1506; MLL3]

other gene names :

[KMT2C; KMT2C; HALR; MLL3; HALR; KIAA1506; MLL3; Lysine N-methyltransferase 2C]

uniprot entry name :

KMT2C_HUMAN

clonality :

Polyclonal

isotype :

host :

Rabbit

reactivity :

Human

sequence length :

4911

specificity :

This MLL3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 3784-3818 amino acids from the C-terminal region of human MLL3.

purity :

Purified

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Immunohistochemistry (IHC) Paraffin, ELISA (EIA)

app notes :

IHC-P:~~1:25

image1 heading :

Immunohistochemistry (IHC)

other info1 :

Cellular Location: Nucleus.

other info2 :

Tissue Location: Highly expressed in testis and ovary, followed by brain and liver. Also expressed in placenta, peripherical blood, fetal thymus, heart, lung and kidney. Within brain, expression was highest in hippocampus, caudate nucleus, and substantia nigra. Not detected in skeletal muscle and fetal liver.

products categories :

New Antibodies

products description :

Histone methyltransferase. Methylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Central component of the MLL2/3 complex, a coactivator complex of nuclear receptors, involved in transcriptional coactivation. KMT2C/MLL3 may be a catalytic subunit of this complex. May be involved in leukemogenesis and developmental disorder.

products references :

Ruault M.,et al.Gene 284:73-81 (2002). Tan Y.C.,et al.Cancer Detect. Prev. 25:454-469 (2001). Hillier L.W.,et al.Nature 424:157-164 (2003). Nagase T.,et al.DNA Res. 7:143-150 (2000). Nakajima D.,et al.DNA Res. 9:99-106 (2002).

ncbi gi num :

91718902

ncbi acc num :

NP_733751.2

ncbi gb acc num :

NM_170606.2

uniprot acc num :

Q8NEZ4

ncbi mol weight :

541370

ncbi pathways :

Lysine Degradation Pathway (82956); Lysine Degradation Pathway (320)

ncbi summary :

This gene is a member of the myeloid/lymphoid or mixed-lineage leukemia (MLL) family and encodes a nuclear protein with an AT hook DNA-binding domain, a DHHC-type zinc finger, six PHD-type zinc fingers, a SET domain, a post-SET domain and a RING-type zinc finger. This protein is a member of the ASC-2/NCOA6 complex (ASCOM), which possesses histone methylation activity and is involved in transcriptional coactivation. [provided by RefSeq, Jul 2008]

uniprot summary :

MLL3: a protein methyltransferase that methylates K4 of histone H3. H3K4 methylation represents a specific tag for epigenetic transcriptional activation. Found in a critical region of chromosome 7, which is commonly deleted in malignant myeloid disorders. Partial duplication of the MLL3 gene are found in the juxtacentromeric region of chromosomes 1, 2, 13 and 21. Central component of the MLL2/3 complex, a coactivator complex of nuclear receptors, involved in transcriptional coactivation. MLL3 may be a catalytic subunit of this complex. Contributes to genome-scale circadian transcription. May be involved in leukemogenesis and developmental disorder. Highly expressed in testis and ovary, followed by brain and liver. Also expressed in placenta, peripherical blood, fetal thymus, heart, lung and kidney. Within brain, expression was highest in hippocampus, caudate nucleus, and substantia nigra. Not detected in skeletal muscle and fetal liver. Belongs to the protein lysine methyltransferase family. TRX/MLL subfamily. Three isoforms of the human protein are produced by alternative splicing. Protein type: EC 2.1.1.43; Methyltransferase; Methyltransferase, protein lysine. Chromosomal Location of Human Ortholog: 7q36.1. Cellular Component: histone methyltransferase complex; nucleoplasm; nucleus. Molecular Function: DNA binding; histone lysine N-methyltransferase activity (H3-K4 specific); histone-lysine N-methyltransferase activity; protein binding; zinc ion binding. Biological Process: histone H3-K4 methylation; regulation of transcription, DNA-dependent; transcription, DNA-dependent

size1 :

0.05 mL

price1 :

150 USD

size2 :

0.2 mL

price2 :

340

more info or order :

MyBioSource product webpage