APOC3 Antibody (C-term) | MyBioSource MBS9217152 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

APOC3 Antibody (C-term)

catalog :

MBS9217152

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-APOC3 Antibody (C-term) at 1:2000 dilution + human plasma lysate. Lysates/proteins at 20 ug per lane. Secondary. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 11 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

image 2 :

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with APOC3 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

product information

catalog number :

MBS9217152

products type :

Antibody

products full name :

APOC3 Antibody (C-term)

products short name :

[APOC3]

products name syn :

[Apolipoprotein C-III; Apo-CIII; ApoC-III; Apolipoprotein C3; APOC3]

other names :

[apolipoprotein C-III; Apolipoprotein C-III; apolipoprotein C-III; apolipoprotein C3; Apolipoprotein C3]

products gene name :

[APOC3]

other gene names :

[APOC3; APOC3; HALP2; APOCIII; Apo-CIII; ApoC-III]

uniprot entry name :

APOC3_HUMAN

clonality :

Polyclonal

isotype :

host :

Rabbit

reactivity :

Human

sequence length :

specificity :

This APOC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70~99 amino acids from the C-term region of human APOC3.

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA)

app notes :

WB:~~1:2000. IHC-P:~~1:10~50

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)-Paraffin

other info1 :

Subtitle: Purified Rabbit Polyclonal Antibody (Pab). Cellular Location: Secreted. Calculated MW: 10852

other info2 :

Tissue Location: Liver. Antigen Region: 70-99

products categories :

Primary Antibodies; Cancer; Cardiovascular; Metabolism; Signal Transduction

products description :

Description: Apolipoprotein C-III is a very low density lipoprotein (VLDL) protein. APOC3 inhibits lipoprotein lipase and hepatic lipase; it is thought to delay catabolism of triglyceride-rich particles. The APOA1, APOC3 and APOA4 genes are closely linked in both rat and human genomes. The A-I and A-IV genes are transcribed from the same strand, while the A-1 and C-III genes are convergently transcribed. An increase in apoC-III levels induces the development of hypertriglyceridemia. Background: Component of triglyceride-rich very low density lipoproteins (VLDL) and high density lipoproteins (HDL) in plasma. Plays a multifaceted role in triglyceride homeostasis. Intracellularly, promotes hepatic very low density lipoprotein 1 (VLDL1) assembly and secretion; extracellularly, attenuates hydrolysis and clearance of triglyceride-rich lipoproteins (TRLs). Impairs the lipolysis of TRLs by inhibiting lipoprotein lipase and the hepatic uptake of TRLs by remnant receptors.

products references :

Liu,Y., Pharmacogenet. Genomics 19 (2), 161-169 (2009). Pollin,T.I., Science 322 (5908), 1702-1705 (2008).

ncbi gi num :

4557323

ncbi acc num :

NP_000031.1

ncbi gb acc num :

NM_000040.1

uniprot acc num :

P02656

ncbi pathways :

Chylomicron-mediated Lipid Transport Pathway (106157); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); HDL-mediated Lipid Transport Pathway (106158); Lipid Digestion, Mobilization, And Transport Pathway (106111); Lipoprotein Metabolism Pathway (106156); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); PPAR Signaling Pathway (83042); PPAR Signaling Pathway (450)

ncbi summary :

Apolipoprotein C-III is a very low density lipoprotein (VLDL) protein. APOC3 inhibits lipoprotein lipase and hepatic lipase; it is thought to delay catabolism of triglyceride-rich particles. The APOA1, APOC3 and APOA4 genes are closely linked in both rat and human genomes. The A-I and A-IV genes are transcribed from the same strand, while the A-1 and C-III genes are convergently transcribed. An increase in apoC-III levels induces the development of hypertriglyceridemia. [provided by RefSeq, Jul 2008]

uniprot summary :

APOC3: Inhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles. Defects in APOC3 are the cause of hyperalphalipoproteinemia type 2 (HALP2). HALP2 is a condition characterized by high levels of high density lipoprotein (HDL) and increased HDL cholesterol levels. Belongs to the apolipoprotein C3 family. Protein type: Secreted; Secreted, signal peptide; Lipid-binding. Chromosomal Location of Human Ortholog: 11q23.3. Cellular Component: chylomicron; early endosome; extracellular region; extracellular space. Molecular Function: cholesterol binding; enzyme regulator activity; lipase inhibitor activity; phospholipid binding. Biological Process: cholesterol efflux; cholesterol homeostasis; G-protein coupled receptor protein signaling pathway; inflammatory response; lipoprotein metabolic process; lipoprotein transport; negative regulation of fatty acid biosynthetic process; negative regulation of lipid catabolic process; negative regulation of lipid metabolic process; negative regulation of lipoprotein lipase activity; negative regulation of receptor-mediated endocytosis; phospholipid efflux; regulation of Cdc42 protein signal transduction; response to peptide hormone stimulus; response to triglyceride; response to vitamin A; retinoid metabolic process; reverse cholesterol transport; triacylglycerol catabolic process; triacylglycerol metabolic process. Disease: Apolipoprotein C-iii Deficiency

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage