EIF2AK3 Antibody | MyBioSource MBS9217062 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

EIF2AK3 Antibody

catalog :

MBS9217062

quantity :

0.05 mL

price :

165 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[1385CT582.4.39]

reactivity :

human, mouse, rat

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

All lanes : Anti-EIF2AK3 Antibody at 1:250-1:1000 dilution. Lane 1: A549 whole cell lysate. Lane 2: PC-12 whole cell lysate. Lane 3: mouse brain lysate. Lane 4: mouse liver lysate. Lysates/proteins at 20 ug per lane. Secondary. Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 125 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

product information

catalog number :

MBS9217062

products type :

Antibody

products full name :

EIF2AK3 Antibody

products short name :

[EIF2AK3]

products name syn :

[Eukaryotic translation initiation factor 2-alpha kinase 3; 2.7.11.1; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK; EIF2AK3; PEK; PERK]

other names :

[eukaryotic translation initiation factor 2-alpha kinase 3 isoform 1; Eukaryotic translation initiation factor 2-alpha kinase 3; eukaryotic translation initiation factor 2-alpha kinase 3; eukaryotic translation initiation factor 2 alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK]

products gene name :

[EIF2AK3]

products gene name syn :

[PEK; PERK]

other gene names :

[EIF2AK3; EIF2AK3; PEK; WRS; PERK; PEK; PERK; HsPEK]

uniprot entry name :

E2AK3_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

[1385CT582.4.39]

host :

Mouse

reactivity :

Human, Mouse, Rat

sequence length :

1116

specificity :

This EIF2AK3 antibody is generated from a mouse immunized with a recombinant protein conjugated synthetic peptide between 530-850 amino acids from human EIF2AK3.

form :

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA)

app notes :

WB:~~1:250-1:1000

image1 heading :

Western Blot (WB)

other info1 :

Cellular Location: Endoplasmic reticulum membrane; Single-pass type I membrane protein.

other info2 :

Function: Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2Sl) on 'Ser-52' during the unfolded protein response (UPR) and in response to low amino acid availability. Converts phosphorylated eIF-2-alpha/EIF2Sl either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Serves as a critical effector of unfolded protein response (UPR)-induced Gl growth arrest due to the loss of cyclin-Dl (CCNDl). Involved in control of mitochondrial morphology and function. Tissue Location: Ubiquitous. A high level expression is seen in secretory tissues

products categories :

New Antibodies; Primary Antibodies; Metabolism

products description :

Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1(CCND1). Involved in control of mitochondrial morphology and function (By similarity).

products references :

Shi Y.,et al.J. Biol. Chem. 274:5723-5730 (1999). Sood R.,et al.Biochem. J. 346:281-293 (2000). Delepine M.,et al.Nat. Genet. 25:406-409 (2000). Ota T.,et al.Nat. Genet. 36:40-45 (2004). Hillier L.W.,et al.Nature 434:724-731 (2005).

ncbi gi num :

134304838

ncbi acc num :

NP_004827.4

ncbi gb acc num :

NM_004836.6

uniprot acc num :

Q9NZJ5

ncbi mol weight :

125216 Da

ncbi pathways :

Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); Hepatitis C Pathway (173973); Hepatitis C Pathway (173907); Herpes Simplex Infection Pathway (377873); Herpes Simplex Infection Pathway (377865); Influenza A Pathway (217173)

ncbi summary :

The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015]

uniprot summary :

PERK: a transmembrane protein kinase of the PEK family resident in the endoplasmic reticulum (ER) membrane and is linked to insulin processing. Couples ER stress to translation inhibition. Stress induces autophosphorylation of its kinase domain and increases its activity. Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (eIF2alpha), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. A critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin d1. Forms dimers with BiP in resting cells. Oligomerizes in ER-stressed cells. LOF mutations cause Wolcott-Rallison syndrome (WRS), characterized by insulin-dependent diabetes in early infancy and, later, multiple system abnormalities. Neuronal death in Alzheimer?s and Parkinson?s diseases is thought to be due to ER stress and has been weakly linked to PEK. Protein type: Kinase, protein; Translation; Membrane protein, integral; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; EC 2.7.11.1; Other group; PEK family; PEK subfamily. Chromosomal Location of Human Ortholog: 2p12. Cellular Component: cytoplasm; endoplasmic reticulum; endoplasmic reticulum membrane; integral to endoplasmic reticulum membrane; membrane; perinuclear region of cytoplasm. Molecular Function: ATP binding; enzyme binding; eukaryotic translation initiation factor 2alpha kinase activity; Hsp90 protein binding; identical protein binding; protein binding; protein homodimerization activity; protein kinase activity; protein phosphatase binding; protein serine/threonine kinase activity. Biological Process: angiogenesis; bone mineralization; calcium-mediated signaling; caspase activation; cellular response to amino acid starvation; cellular response to glucose starvation; chondrocyte development; endocrine pancreas development; endoplasmic reticulum organization and biogenesis; ER overload response; insulin secretion; insulin-like growth factor receptor signaling pathway; negative regulation of myelination; negative regulation of translation; negative regulation of translation initiation in response to stress; ossification; peptidyl-serine phosphorylation; positive regulation of transcription from RNA polymerase I promoter; protein amino acid autophosphorylation; protein amino acid phosphorylation; protein homooligomerization; skeletal development; unfolded protein response. Disease: Epiphyseal Dysplasia, Multiple, With Early-onset Diabetes Mellitus

size1 :

0.05 mL

price1 :

165 USD

size2 :

0.2 mL

price2 :

370

more info or order :

MyBioSource product webpage