WNT4 Antibody | MyBioSource MBS9217056 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

WNT4 Antibody

catalog :

MBS9217056

quantity :

0.05 mL

price :

165 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[1698CT540.169.185]

reactivity :

human, mouse

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

All lanes : Anti-WNT4 Antibody at 1:4000 dilution. Lane 1: Hela whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lane 3: mouse lung lysate. Lane 4: SK-BR-3 whole cell lysate. Lane 5: MDA-MB-231 whole cell lysate. Lysates/proteins at 20 ug per lane. Secondary. Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 39 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

product information

catalog number :

MBS9217056

products type :

Antibody

products full name :

WNT4 Antibody

products short name :

[WNT4]

products name syn :

[Protein Wnt-4; WNT4]

other names :

[protein Wnt-4; Protein Wnt-4; protein Wnt-4; Wnt family member 4]

products gene name :

[WNT4]

other gene names :

[WNT4; WNT4; WNT-4; SERKAL]

uniprot entry name :

WNT4_HUMAN

clonality :

Monoclonal

isotype :

IgG1,k

clone :

[1698CT540.169.185]

host :

Mouse

reactivity :

Human, Mouse

sequence length :

351

specificity :

This WNT4 antibody is generated from a mouse immunized with a recombinant protein between 30-351 amino acids from human WNT4.

form :

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA)

app notes :

WB:~~1:4000

image1 heading :

Western Blot (WB)

other info1 :

Cellular Location: Secreted, extracellular space, extracellular matrix.

products categories :

Crown Antibodies; New Antibodies; Primary Antibodies; Cancer; Developmental Biology; Signal Transduction; Stem Cells

products description :

Background: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity). Overexpression may be associated with abnormal proliferation in human breast tissue. Function: Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Plays an important role in the embryonic development of the urogenital tract and the lung (PubMed: 15317892 , PubMed: 16959810 , PubMed: 18179883 , PubMed: 18182450 ). Required for normal mesenchyme to epithelium transition during embryonic kidney development. Required for the formation of early epithelial renal vesicles during kidney development (By similarity). Required for normal formation of the Mullerian duct in females, and normal levels of oocytes in the ovaries (PubMed: 15317892 , PubMed: 16959810 , PubMed: 18182450 ). Required for normal down-regulation of 3 beta-hydroxysteroid dehydrogenase in the ovary (PubMed: 15317892 , PubMed: 16959810 , PubMed: 18182450 ). Requiredfor normal lung development and for normal patterning of trachael cartilage rings (By similarity).

products references :

Testa T.T.,et al.Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases. Jordan B.K.,et al.Am. J. Hum. Genet. 68:1102-1109 (2001). Clark H.F.,et al.Genome Res. 13:2265-2270 (2003). Ota T.,et al.Nat. Genet. 36:40-45 (2004). Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.

ncbi gi num :

17402922

ncbi acc num :

NP_110388.2

ncbi gb acc num :

NM_030761.4

uniprot acc num :

P56705

ncbi pathways :

Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); Class B/2 (Secretin Family Receptors) Pathway (106378); DNA Damage Response (only ATM Dependent) Pathway (198827); GPCR Ligand Binding Pathway (161020); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889); Hedgehog Signaling Pathway (83063); Hedgehog Signaling Pathway (474); Hippo Signaling Pathway (749777)

ncbi summary :

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and is the first signaling molecule shown to influence the sex-determination cascade. It encodes a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. This gene and a nuclear receptor known to antagonize the testis-determining factor play a concerted role in both the control of female development and the prevention of testes formation. This gene and another two family members, WNT2 and WNT7B, may be associated with abnormal proliferation in breast tissue. Mutations in this gene can result in Rokitansky-Kuster-Hauser syndrome and in SERKAL syndrome. [provided by RefSeq, Jul 2008]

uniprot summary :

WNT4: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Overexpression may be associated with abnormal proliferation in human breast tissue. Defects in WNT4 are a cause of Rokitansky-Kuster-Hauser syndrome (RKH syndrome); also called Mayer- Rokitansky-Kuster-Hauser syndrome (MRKH syndrome or MRKH anomaly). RKH syndrome is characterized by utero-vaginal atresia in otherwise phenotypically normal female with a normal 46,XX karyotype. Anomalies of the genital tract range from upper vaginal atresia to total Muellerian agenesis with urinary tract abnormalities. It has an incidence of approximately 1 in 5'000 newborn girls. Defects in WNT4 are the cause of female sex reversal with dysgenesis of kidneys, adrenals, and lungs (SERKAL); also known as SERKAL syndrome. Defects in WNT4 are the cause of Muellerian aplasia (MULLAPL). Belongs to the Wnt family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 1p36.23-p35.1. Cellular Component: cell surface; cytoplasm; endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi lumen; plasma membrane; proteinaceous extracellular matrix. Molecular Function: frizzled binding; receptor agonist activity; transcription corepressor activity. Biological Process: adrenal gland development; androgen biosynthetic process; cell fate commitment; cellular response to starvation; embryonic epithelial tube formation; epithelial to mesenchymal transition; female gonad development; female sex determination; immature T cell proliferation in the thymus; kidney development; liver development; male gonad development; negative regulation of cell differentiation; negative regulation of cell migration; negative regulation of fibroblast growth factor receptor signaling pathway; negative regulation of transcription, DNA-dependent; neuron differentiation; oocyte development; positive regulation of aldosterone biosynthetic process; positive regulation of bone mineralization; positive regulation of collagen biosynthetic process; positive regulation of focal adhesion formation; positive regulation of GTPase activity; positive regulation of meiosis; positive regulation of osteoblast differentiation; positive regulation of stress fiber formation; positive regulation of transcription, DNA-dependent; regulation of cell-cell adhesion; smooth muscle cell differentiation; somatotropin secreting cell differentiation; thyroid stimulating hormone secreting cell differentiation; ureteric bud branching; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin. Disease: 46,xx Sex Reversal With Dysgenesis Of Kidneys, Adrenals, And Lungs; Mullerian Aplasia And Hyperandrogenism

size1 :

0.05 mL

price1 :

165 USD

size2 :

0.2 mL

price2 :

370

more info or order :

MyBioSource product webpage